Detailed information    

insolico Bioinformatically predicted

Overview

Name   recD2   Type   Machinery gene
Locus tag   I8N72_RS12780 Genome accession   NZ_CP065790
Coordinates   2626994..2629372 (-) Length   792 a.a.
NCBI ID   WP_014305491.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM1082     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2600062..2646541 2626994..2629372 within 0


Gene organization within MGE regions


Location: 2600062..2646541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N72_RS12585 (I8N72_12585) - 2600450..2600755 (-) 306 WP_015387929.1 hypothetical protein -
  I8N72_RS12590 (I8N72_12590) - 2600755..2601354 (-) 600 WP_015387928.1 dUTP diphosphatase -
  I8N72_RS12595 (I8N72_12595) - 2601351..2601674 (-) 324 WP_015387927.1 hypothetical protein -
  I8N72_RS12600 (I8N72_12600) - 2601784..2602608 (-) 825 WP_015387925.1 DNA adenine methylase -
  I8N72_RS12605 (I8N72_12605) - 2602622..2603998 (-) 1377 WP_015387924.1 DNA cytosine methyltransferase -
  I8N72_RS12610 (I8N72_12610) - 2604003..2604260 (-) 258 WP_015387923.1 hypothetical protein -
  I8N72_RS12615 (I8N72_12615) - 2604273..2604674 (-) 402 WP_015387922.1 hypothetical protein -
  I8N72_RS12620 (I8N72_12620) - 2604671..2604901 (-) 231 WP_015387921.1 hypothetical protein -
  I8N72_RS12625 (I8N72_12625) - 2604898..2605281 (-) 384 WP_015387920.1 hypothetical protein -
  I8N72_RS12630 (I8N72_12630) - 2605422..2605625 (-) 204 WP_014721584.1 XtrA/YqaO family protein -
  I8N72_RS12635 (I8N72_12635) - 2605705..2605860 (-) 156 WP_015387919.1 hypothetical protein -
  I8N72_RS12640 (I8N72_12640) - 2605873..2606013 (-) 141 WP_015387918.1 BH0509 family protein -
  I8N72_RS12645 (I8N72_12645) - 2606121..2606669 (-) 549 WP_015387916.1 hypothetical protein -
  I8N72_RS12650 (I8N72_12650) - 2606666..2606824 (-) 159 WP_015387915.1 DUF6906 family protein -
  I8N72_RS12655 (I8N72_12655) - 2606821..2606970 (-) 150 WP_015387914.1 hypothetical protein -
  I8N72_RS12660 (I8N72_12660) - 2606985..2607818 (-) 834 WP_031306578.1 ATP-binding protein -
  I8N72_RS12665 (I8N72_12665) - 2607802..2608674 (-) 873 WP_015387912.1 conserved phage C-terminal domain-containing protein -
  I8N72_RS12670 (I8N72_12670) - 2608688..2609005 (-) 318 WP_015387911.1 hypothetical protein -
  I8N72_RS12675 (I8N72_12675) - 2609046..2609237 (-) 192 WP_015387910.1 helix-turn-helix domain-containing protein -
  I8N72_RS12680 (I8N72_12680) - 2609407..2609796 (+) 390 WP_015387909.1 helix-turn-helix domain-containing protein -
  I8N72_RS12685 (I8N72_12685) - 2610167..2611348 (-) 1182 WP_014418511.1 AimR family lysis-lysogeny pheromone receptor -
  I8N72_RS12690 (I8N72_12690) - 2611655..2612815 (+) 1161 WP_015387907.1 site-specific integrase -
  I8N72_RS12695 (I8N72_12695) udk 2612880..2613512 (-) 633 WP_014418513.1 uridine kinase -
  I8N72_RS12700 (I8N72_12700) - 2613519..2614787 (-) 1269 WP_014305485.1 peptidase U32 family protein -
  I8N72_RS12705 (I8N72_12705) - 2614805..2615734 (-) 930 WP_003152757.1 peptidase U32 family protein -
  I8N72_RS12710 (I8N72_12710) - 2615741..2616394 (-) 654 WP_015387906.1 O-methyltransferase -
  I8N72_RS12715 (I8N72_12715) mltG 2616546..2617637 (-) 1092 WP_014305487.1 endolytic transglycosylase MltG -
  I8N72_RS12720 (I8N72_12720) - 2617748..2618029 (-) 282 WP_003152751.1 DUF1292 domain-containing protein -
  I8N72_RS12725 (I8N72_12725) ruvX 2618042..2618458 (-) 417 WP_003152750.1 Holliday junction resolvase RuvX -
  I8N72_RS12730 (I8N72_12730) - 2618467..2618733 (-) 267 WP_003152749.1 IreB family regulatory phosphoprotein -
  I8N72_RS12735 (I8N72_12735) alaS 2618818..2621454 (-) 2637 WP_003152748.1 alanine--tRNA ligase -
  I8N72_RS12740 (I8N72_12740) - 2621786..2622847 (-) 1062 WP_015387905.1 AI-2E family transporter -
  I8N72_RS12745 (I8N72_12745) - 2623067..2623795 (+) 729 WP_003152746.1 amino acid ABC transporter ATP-binding protein -
  I8N72_RS12750 (I8N72_12750) - 2623816..2624643 (+) 828 WP_003152745.1 transporter substrate-binding domain-containing protein -
  I8N72_RS12755 (I8N72_12755) - 2624700..2625350 (+) 651 WP_003152744.1 amino acid ABC transporter permease -
  I8N72_RS12760 (I8N72_12760) - 2625368..2626024 (+) 657 WP_015387904.1 amino acid ABC transporter permease -
  I8N72_RS12765 (I8N72_12765) - 2626060..2626191 (-) 132 WP_003152741.1 YrzQ family protein -
  I8N72_RS12770 (I8N72_12770) - 2626213..2626404 (-) 192 WP_003152738.1 hypothetical protein -
  I8N72_RS12775 (I8N72_12775) - 2626417..2626875 (-) 459 WP_003152735.1 PRC-barrel domain-containing protein -
  I8N72_RS12780 (I8N72_12780) recD2 2626994..2629372 (-) 2379 WP_014305491.1 ATP-dependent RecD-like DNA helicase Machinery gene
  I8N72_RS12785 (I8N72_12785) - 2629391..2630011 (-) 621 WP_088005508.1 tetratricopeptide repeat protein -
  I8N72_RS12790 (I8N72_12790) mnmA 2630099..2631214 (-) 1116 WP_015387903.1 tRNA 2-thiouridine(34) synthase MnmA -
  I8N72_RS12795 (I8N72_12795) - 2631251..2632390 (-) 1140 WP_003152731.1 cysteine desulfurase family protein -
  I8N72_RS12800 (I8N72_12800) cymR 2632409..2632825 (-) 417 WP_003152730.1 cysteine metabolism transcriptional regulator CymR -
  I8N72_RS12805 (I8N72_12805) - 2633020..2634288 (+) 1269 WP_015387902.1 replication-associated recombination protein A -
  I8N72_RS12810 (I8N72_12810) - 2634364..2635128 (-) 765 WP_003152728.1 ThiF family adenylyltransferase -
  I8N72_RS12820 (I8N72_12820) aspS 2635455..2637233 (-) 1779 WP_015387901.1 aspartate--tRNA ligase -
  I8N72_RS12825 (I8N72_12825) hisS 2637248..2638522 (-) 1275 WP_003152726.1 histidine--tRNA ligase -
  I8N72_RS12830 (I8N72_12830) - 2638879..2639049 (-) 171 WP_003152722.1 YrzK family protein -
  I8N72_RS12835 (I8N72_12835) - 2639180..2640742 (+) 1563 WP_015387900.1 SH3 domain-containing protein -
  I8N72_RS12840 (I8N72_12840) dtd 2640769..2641212 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  I8N72_RS12845 (I8N72_12845) relA 2641225..2643429 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  I8N72_RS12850 (I8N72_12850) - 2643587..2644099 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  I8N72_RS12855 (I8N72_12855) recJ 2644105..2646465 (-) 2361 WP_015387899.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene

Sequence

Protein


Download         Length: 792 a.a.        Molecular weight: 88334.01 Da        Isoelectric Point: 4.9490

>NTDB_id=514487 I8N72_RS12780 WP_014305491.1 2626994..2629372(-) (recD2) [Bacillus velezensis strain LBUM1082]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILADASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDEAFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQIKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide


Download         Length: 2379 bp        

>NTDB_id=514487 I8N72_RS12780 WP_014305491.1 2626994..2629372(-) (recD2) [Bacillus velezensis strain LBUM1082]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAGGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTACGGAAAAACTGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGAATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTTGGAGACGGCGCGCTCAATAAAATTCTGGCTGATGCGTCGGTGCTTT
ATGATGTGCCGAAAATGTCAAAAAAGAAAGCGGACAAACTGGCGGGAGAGCTGCAGCGGCATCAGGGGCTTGAACAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCCATGAAAATATATCAGGCCTACGAAACGGAAACCTTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGCAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAG
GGCCACACGTACATAGAAACGAAGCAGCTGATTATCAATACGCAAACGCTTCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAAATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATCGAAGGAGAGCGCTGCTATT
TTCCCTCCTTGTTTTACGCGGAACAAAACGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTACTGACCGGGGGACCTGGAACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGCGTATCCCTTGACCCCGGTGATTATAAAAAAGACGAGGCGTTTCCGGTG
CTGCTTGCCGCGCCGACCGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGTCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAACGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTTGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTTGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGATCTTTTGGCTTCTGAAGTGCTGCCGACCGTGCGCCT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCCCATGATATGAAAAAAGGACTGCTTCCAAAGG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATTAAAGAAGTCGTGGATCAGGTCGTC
TCGAATGCGTTAAAAAAGGGGTATACCTCCAGAGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAGGACATCATGAATCCGCCTAAAGAAAAAAGGCGGGAGCTGAAATTCGGAGATGTCGTCT
ACAGGACGGGCGATAAAATATTGCAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAACGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGGAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATTACAAGAGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCTTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix containing domain (HHH_RecD2)

(154-244)

ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13)

(589-659)

AAA domain (AAA_30)

(341-548)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(676-724)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recD2 Bacillus subtilis subsp. subtilis str. 168

89.001

99.874

0.889