Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I8N72_RS08820 Genome accession   NZ_CP065790
Coordinates   1806374..1806808 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus velezensis strain LBUM1082     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1801374..1811808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8N72_RS08815 (I8N72_08815) - 1804874..1806085 (-) 1212 WP_003154086.1 cytochrome P450 family protein -
  I8N72_RS08820 (I8N72_08820) nucA/comI 1806374..1806808 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  I8N72_RS08825 (I8N72_08825) - 1806868..1807623 (+) 756 WP_003154084.1 YoaK family protein -
  I8N72_RS08830 (I8N72_08830) - 1807657..1808019 (-) 363 WP_003154082.1 hypothetical protein -
  I8N72_RS08835 (I8N72_08835) - 1808211..1809539 (-) 1329 WP_003154079.1 S8 family peptidase -
  I8N72_RS08840 (I8N72_08840) - 1809719..1809952 (+) 234 WP_031306556.1 hypothetical protein -
  I8N72_RS08845 (I8N72_08845) - 1810208..1810915 (+) 708 WP_015388206.1 poly-gamma-glutamate hydrolase family protein -
  I8N72_RS08850 (I8N72_08850) - 1810976..1811428 (+) 453 WP_015388205.1 OsmC family protein -
  I8N72_RS08855 (I8N72_08855) - 1811442..1811795 (-) 354 WP_003154073.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=514471 I8N72_RS08820 WP_003154085.1 1806374..1806808(+) (nucA/comI) [Bacillus velezensis strain LBUM1082]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=514471 I8N72_RS08820 WP_003154085.1 1806374..1806808(+) (nucA/comI) [Bacillus velezensis strain LBUM1082]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486