Detailed information NTG0514261

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: STMM8_RS04305
• Genome accession: NZ_CP065787
• Coordinates: 810428..811267 (+)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus strain MM8
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 805428..816267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
STMM8_RS04275 (STMM8_04260)	-	805844..806371 (+)	528	WP_022096553.1	ECF transporter S component	-
STMM8_RS04280 (STMM8_04265)	-	806395..806910 (+)	516	WP_011681082.1	transcription repressor NadR	-
STMM8_RS04285 (STMM8_04270)	-	807005..807694 (+)	690	WP_011225885.1	helix-turn-helix transcriptional regulator	-
STMM8_RS04290 (STMM8_04275)	ylqF	807822..808676 (+)	855	WP_022096554.1	ribosome biogenesis GTPase YlqF	-
STMM8_RS04295 (STMM8_04280)	-	808663..809430 (+)	768	WP_002946264.1	ribonuclease HII	-
STMM8_RS04300 (STMM8_04285)	-	809441..810360 (+)	920	Protein_788	ROK family protein	-
STMM8_RS04305 (STMM8_04290)	dprA	810428..811267 (+)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
STMM8_RS04310 (STMM8_04295)	topA	811435..813579 (+)	2145	WP_022096555.1	type I DNA topoisomerase	-
STMM8_RS04315 (STMM8_04300)	-	813697..814521 (+)	825	WP_022096556.1	TraX family protein	-
STMM8_RS04320 (STMM8_04305)	-	814557..815216 (+)	660	WP_022096557.1	response regulator transcription factor	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=514261 STMM8_RS04305 WP_011225887.1 810428..811267(+) (dprA) [Streptococcus thermophilus strain MM8]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=514261 STMM8_RS04305 WP_011225887.1 810428..811267(+) (dprA) [Streptococcus thermophilus strain MM8]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTAGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTGAAACATTCCTATAATCCTCCTGTCCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGTGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362