Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   KUU77_RS25705 Genome accession   NZ_CP077985
Coordinates   5456705..5457130 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain ZPPH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5451705..5462130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KUU77_RS25690 (KUU77_25675) pilX 5452269..5452856 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  KUU77_RS25695 (KUU77_25680) pilY1 5452868..5456359 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  KUU77_RS25700 (KUU77_25685) pilY2 5456361..5456708 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  KUU77_RS25705 (KUU77_25690) comF 5456705..5457130 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  KUU77_RS25710 (KUU77_25695) ispH 5457177..5458121 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  KUU77_RS25715 (KUU77_25700) fkpB 5458207..5458647 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  KUU77_RS25720 (KUU77_25705) lspA 5458640..5459149 (-) 510 WP_003094728.1 signal peptidase II -
  KUU77_RS25725 (KUU77_25710) ileS 5459142..5461973 (-) 2832 WP_222821464.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=512488 KUU77_RS25705 WP_003094721.1 5456705..5457130(+) (comF) [Pseudomonas aeruginosa strain ZPPH2]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=512488 KUU77_RS25705 WP_003094721.1 5456705..5457130(+) (comF) [Pseudomonas aeruginosa strain ZPPH2]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383