Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   I4W80_RS05295 Genome accession   NZ_CP065591
Coordinates   1047124..1050633 (+) Length   1169 a.a.
NCBI ID   WP_098680882.1    Uniprot ID   -
Organism   Bacillus altitudinis strain 1817     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1042124..1055633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I4W80_RS05265 (I4W80_05265) lepB 1042630..1043142 (+) 513 WP_007499139.1 signal peptidase I -
  I4W80_RS05270 (I4W80_05270) - 1043178..1044320 (-) 1143 WP_007499137.1 AbrB family transcriptional regulator -
  I4W80_RS05275 (I4W80_05275) - 1044597..1045019 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  I4W80_RS05280 (I4W80_05280) - 1045012..1045851 (+) 840 WP_206116121.1 M56 family metallopeptidase -
  I4W80_RS05285 (I4W80_05285) - 1045909..1046319 (+) 411 WP_008348626.1 DoxX family protein -
  I4W80_RS05290 (I4W80_05290) - 1046511..1046957 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  I4W80_RS05295 (I4W80_05295) addB 1047124..1050633 (+) 3510 WP_098680882.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  I4W80_RS05300 (I4W80_05300) addA 1050611..1054315 (+) 3705 WP_206116122.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  I4W80_RS05305 (I4W80_05305) sbcD 1054341..1055516 (+) 1176 WP_007499123.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134621.49 Da        Isoelectric Point: 5.1659

>NTDB_id=512101 I4W80_RS05295 WP_098680882.1 1047124..1050633(+) (addB) [Bacillus altitudinis strain 1817]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILALVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVVQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYVSYSASDTEGRSLLPSTLIKYCEELMPNHQQALYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNRDVSNKIMSSLFFTNRATRLNPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLAEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=512101 I4W80_RS05295 WP_098680882.1 1047124..1050633(+) (addB) [Bacillus altitudinis strain 1817]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTTCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CATCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGAATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGATTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAAATCTCGGCATCCAGTATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGGGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCAGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGTTGGCCAA
AGAGTGCGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGCTTTAGTAAGAGAGGAAGGTCTTAGGTTACGAGATATCTCAAT
CGTTGCAAGACATGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGCTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCTAAAAACAAAGCGAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGCTTTCATTATCGCCGCT
TTCAATCATTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCACTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAGGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGCTTGAGGCAGAAGAAGCAGGCAGATTAGCTGAAG
CGATGCAGCATGGGCAAGTGTGGGATGCTGTCGTTCAATTGATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCATTATTTCAACAGATGATGGATACAGGACTGTCCTCTCTCAAGTTCGCTTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGAGTTA
TACCTGCACGGCCCTCTGATGAAAGTGTATTGTCTGAAGACGACCGGGAATGGTTAAAACGTGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATTTATCAAGCGCTGTCCAGTCCATCGCACTACCTGTATGT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTCTTTTGCCTTCGACCCTCATCAAGTATTGTGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGATGATCAGGAGCAATTAAAATTTGTAACAAACGAGCAT
GTCTCGTTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACCGCGACGTATCAAATAAAATCATGTCCAGCTTGTTCTTTACAAATAGAGCGACAC
GTTTAAATCCGAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGTTAAAACTAAAAGATCGACAATTTTACAAATTAGAAGCACCTGATATCGG
TCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCATGCAATCGAACAGCTGGCTCCACGTCTGCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATCAAAGAAAAGCTCCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCCTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAAGCGACACCGGCAGGGATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCGCCTATTCCGCTCGCAGAGGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAAATAAACCCATATAAATTAAAGGATCAAACCCCT
TGCCGCTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACCTCTGA
GAAGGATGATGTCATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.581

99.316

0.661