Detailed information NTG0511321

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: ST4147_RS05425
• Genome accession: NZ_CP065504
• Coordinates: 1024963..1025844 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus strain 4147
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1019963..1030844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST4147_RS05400 (ST4147_05425)	vicR	1020663..1021370 (-)	708	WP_011227274.1	response regulator YycF	Regulator
ST4147_RS05405 (ST4147_05430)	-	1021902..1022669 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
ST4147_RS05410 (ST4147_05435)	-	1022681..1023514 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
ST4147_RS05415 (ST4147_05440)	-	1023532..1024230 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
ST4147_RS05420 (ST4147_05445)	-	1024242..1024892 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
ST4147_RS05425 (ST4147_05450)	endA	1024963..1025844 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
ST4147_RS05430 (ST4147_05455)	-	1025906..1026094 (-)	189	WP_011227277.1	DNA-directed RNA polymerase subunit beta	-
ST4147_RS05435 (ST4147_05460)	murA	1026096..1027367 (-)	1272	WP_041827046.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
ST4147_RS05440 (ST4147_05465)	-	1027437..1027673 (-)	237	WP_002946959.1	DUF1146 family protein	-
ST4147_RS05445 (ST4147_05470)	galE	1027861..1028874 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
ST4147_RS05450 (ST4147_05475)	-	1028889..1029344 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=511321 ST4147_RS05425 WP_011226089.1 1024963..1025844(-) (endA) [Streptococcus thermophilus strain 4147]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=511321 ST4147_RS05425 WP_011226089.1 1024963..1025844(-) (endA) [Streptococcus thermophilus strain 4147]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594