Detailed information NTG0510250

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: ST90730_RS05375
• Genome accession: NZ_CP065481
• Coordinates: 1019166..1020047 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus strain 90730
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1014166..1025047

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST90730_RS05350 (ST90730_05385)	vicR	1014866..1015573 (-)	708	WP_011227274.1	response regulator YycF	Regulator
ST90730_RS05355 (ST90730_05390)	-	1016105..1016872 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
ST90730_RS05360 (ST90730_05395)	-	1016884..1017717 (+)	834	WP_011227276.1	transporter substrate-binding domain-containing protein	-
ST90730_RS05365 (ST90730_05400)	-	1017735..1018433 (+)	699	WP_011226087.1	amino acid ABC transporter permease	-
ST90730_RS05370 (ST90730_05405)	-	1018445..1019095 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
ST90730_RS05375 (ST90730_05410)	endA	1019166..1020047 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
ST90730_RS05380 (ST90730_05415)	-	1020109..1020297 (-)	189	WP_011227277.1	DNA-directed RNA polymerase subunit beta	-
ST90730_RS05385 (ST90730_05420)	murA	1020299..1021570 (-)	1272	WP_041827046.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
ST90730_RS05390 (ST90730_05425)	-	1021640..1021876 (-)	237	WP_002946959.1	DUF1146 family protein	-
ST90730_RS05395 (ST90730_05430)	galE	1022064..1023077 (-)	1014	WP_011227279.1	UDP-glucose 4-epimerase GalE	-
ST90730_RS05400 (ST90730_05435)	-	1023092..1023547 (-)	456	WP_041827047.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=510250 ST90730_RS05375 WP_011226089.1 1019166..1020047(-) (endA) [Streptococcus thermophilus strain 90730]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=510250 ST90730_RS05375 WP_011226089.1 1019166..1020047(-) (endA) [Streptococcus thermophilus strain 90730]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCCCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594