Detailed information NTG0510130

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: ST90728_RS05690
• Genome accession: NZ_CP065479
• Coordinates: 1089850..1090731 (-)
• Length: 293 a.a.
• NCBI ID: WP_011226089.1
• Uniprot ID: Q5M441
• Organism: Streptococcus thermophilus strain 90728
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1084850..1095731

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ST90728_RS05665 (ST90728_05670)	vicR	1085554..1086261 (-)	708	WP_173940375.1	response regulator YycF	Regulator
ST90728_RS05670 (ST90728_05675)	-	1086788..1087555 (+)	768	WP_011227275.1	amino acid ABC transporter ATP-binding protein	-
ST90728_RS05675 (ST90728_05680)	-	1087567..1088400 (+)	834	WP_002953142.1	transporter substrate-binding domain-containing protein	-
ST90728_RS05680 (ST90728_05685)	-	1088418..1089116 (+)	699	WP_065973256.1	amino acid ABC transporter permease	-
ST90728_RS05685 (ST90728_05690)	-	1089128..1089778 (+)	651	WP_011226088.1	amino acid ABC transporter permease	-
ST90728_RS05690 (ST90728_05695)	endA	1089850..1090731 (-)	882	WP_011226089.1	DNA/RNA non-specific endonuclease	Machinery gene
ST90728_RS05695 (ST90728_05700)	-	1090793..1090981 (-)	189	WP_011226090.1	DNA-directed RNA polymerase subunit beta	-
ST90728_RS05700 (ST90728_05705)	murA	1090983..1092254 (-)	1272	WP_179970494.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
ST90728_RS05705 (ST90728_05710)	-	1092324..1092560 (-)	237	WP_002946959.1	DUF1146 family protein	-
ST90728_RS05710 (ST90728_05715)	galE	1092748..1093761 (-)	1014	WP_002946961.1	UDP-glucose 4-epimerase GalE	-
ST90728_RS05715	-	1093994..1094392 (-)	399	WP_332068245.1	DUF4832 domain-containing protein	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31757.50 Da
• Isoelectric Point: 10.1789

>NTDB_id=510130 ST90728_RS05690 WP_011226089.1 1089850..1090731(-) (endA) [Streptococcus thermophilus strain 90728]
MAKRKTSLTNKQKRQLVTLIIAALIAVLGYLGTSNKLSPDNPIRQVASLVAGKSNKSIKSNGSTNSQSTPQEQLAETVMT
SSVKSQLGNSLEWNGAGAYIINRNKTNLNAKVSSQPYANNQVKTVQGQTVPTVANALLSKATRQYKNRQETGNGSTSWTP
AGWHQVQNLSGEYSHAVDRGHLLGYALIGGLKGFDASTSNPENIAVQTAWANQAYSDSSTGQNYYESLVRKALDKNKRVR
YRVTLIYEGENLIPSGTHLEAKSADGSLEFNVFVPNVQEGITLDYYSGKVTVN

Nucleotide

• Length: 882 bp

>NTDB_id=510130 ST90728_RS05690 WP_011226089.1 1089850..1090731(-) (endA) [Streptococcus thermophilus strain 90728]
ATGGCAAAAAGAAAGACATCTTTGACAAATAAACAGAAACGTCAGTTAGTCACATTAATAATAGCAGCTTTGATAGCTGT
TTTAGGTTACCTAGGAACCAGTAACAAGCTCTCGCCTGATAATCCTATCAGACAAGTAGCAAGCCTAGTTGCAGGGAAAT
CTAACAAATCCATTAAATCTAATGGTTCAACAAATAGTCAGTCTACACCACAAGAGCAGCTTGCTGAGACAGTAATGACT
AGTTCAGTCAAAAGCCAACTGGGTAATTCCTTGGAATGGAATGGAGCTGGGGCCTACATTATTAATAGAAATAAGACGAA
TCTAAATGCTAAGGTATCAAGCCAACCCTATGCTAATAACCAAGTCAAAACGGTTCAAGGACAAACTGTTCCTACAGTTG
CTAATGCCTTATTAAGTAAAGCAACTAGACAGTACAAAAATCGTCAAGAAACAGGGAATGGTTCAACCTCTTGGACACCA
GCTGGATGGCACCAGGTTCAAAATTTATCAGGAGAGTATAGCCATGCCGTAGATAGAGGCCATTTGCTAGGTTATGCACT
GATAGGTGGTTTGAAAGGATTTGATGCCTCGACTTCAAATCCAGAGAATATTGCTGTTCAAACTGCTTGGGCAAATCAAG
CATATAGCGATAGTTCTACAGGCCAAAACTATTATGAAAGCCTAGTACGAAAAGCTCTGGATAAAAACAAACGTGTTCGT
TATCGTGTGACCCTGATTTATGAAGGAGAGAATCTCATTCCATCAGGAACACATCTTGAAGCTAAGTCAGCAGACGGCTC
ACTTGAGTTTAATGTCTTTGTTCCAAATGTACAAGAGGGAATTACACTAGATTACTATTCAGGAAAAGTAACGGTGAATT
GA

Domains

Predicted by InterproScan.

• DNA/RNA non-specific endonuclease (Endonuclease_NS) (101-266)

3D structure

• AlphaFold DB: Q5M441

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	72.803	81.57	0.594
endA	Streptococcus pneumoniae D39	72.803	81.57	0.594
endA	Streptococcus pneumoniae R6	72.803	81.57	0.594
endA	Streptococcus pneumoniae TIGR4	72.803	81.57	0.594