Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   KU528_RS12275 Genome accession   NZ_CP077865
Coordinates   2487401..2489749 (+) Length   782 a.a.
NCBI ID   WP_001249291.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 370     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2482401..2494749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU528_RS12255 (KU528_12205) rnhC 2483505..2484443 (-) 939 WP_001284269.1 ribonuclease HIII -
  KU528_RS12260 (KU528_12210) zapA 2484818..2485077 (+) 260 Protein_2375 cell division protein ZapA -
  KU528_RS12265 (KU528_12215) - 2485085..2485606 (+) 522 WP_000234071.1 CvpA family protein -
  KU528_RS12270 (KU528_12220) polX 2485679..2487391 (+) 1713 WP_000161946.1 DNA polymerase/3'-5' exonuclease PolX -
  KU528_RS12275 (KU528_12225) mutS/mutS2 2487401..2489749 (+) 2349 WP_001249291.1 endonuclease MutS2 Machinery gene
  KU528_RS12280 (KU528_12230) trxA 2489922..2490236 (+) 315 WP_001018928.1 thioredoxin -
  KU528_RS12285 (KU528_12235) - 2490299..2490394 (-) 96 WP_031844936.1 hypothetical protein -
  KU528_RS12290 (KU528_12240) uvrC 2490560..2492341 (+) 1782 WP_000390527.1 excinuclease ABC subunit UvrC Machinery gene
  KU528_RS12295 (KU528_12245) - 2492665..2493279 (+) 615 WP_000079317.1 succinate dehydrogenase cytochrome b558 subunit -

Sequence

Protein


Download         Length: 782 a.a.        Molecular weight: 88638.28 Da        Isoelectric Point: 6.0352

>NTDB_id=509090 KU528_RS12275 WP_001249291.1 2487401..2489749(+) (mutS/mutS2) [Staphylococcus aureus strain 370]
MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVVFQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADI
GGVLNVSELNLIKRLIQVQNQFKTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQSASGQTLYIEPSSVVEMNNQISR
LRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQLDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVA
NTIEFMEDIETVIITGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLSTFSSHMTNI
VEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTHYPELKAYSYNREGVMNASVEFDVDTLSPTY
KLLMGVPGRSNAFDISKKLGLSLNIINKAKTMIGTDEKEINEMIESLERNYKRVETQRLELDCLVKEAEQVHDDLSKQYQ
QFQNYEKSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEAKSIKQNVQKQKYDKIV
AGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLEKKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIE
LDQYLDQAVLSNYEQVYIIHGKGTGALQKGVQQHLKKHKCVSDFRGGMPSEGGFGVTVATLK

Nucleotide


Download         Length: 2349 bp        

>NTDB_id=509090 KU528_RS12275 WP_001249291.1 2487401..2489749(+) (mutS/mutS2) [Staphylococcus aureus strain 370]
ATGAGACAAAAAACATTAGACGTCTTAGAATTTGAAAAAATAAAATCACTCGTTGCCAATGAAACAATTAGTGACTTAGG
CTTGGAAAAGGTCAATCAAATGATGCCAGCTACTAATTTTGAAACGGTTGTTTTTCAAATGGAAGAAACGGATGAGATTG
CTCAAATCTATAATAAGCATCGTTTACCAAGCTTGAGTGGCTTATCTAAAGTATCAGCATTCATTCATCGCGCTGATATT
GGCGGCGTTTTAAATGTATCAGAGCTTAACTTGATAAAAAGATTAATTCAAGTACAAAATCAATTTAAGACATTTTATAA
TCAATTGGTTGAAGAAGATGAAGGTGTTAAATACCCAATATTAGATGACAAGATGAATCAATTACCTGTGTTAACAGATC
TTTTTCAACAAATAAATGAAACATGTGATACGTACGATTTATATGATAATGCGAGTTATGAATTGCAAGGGATTAGAAGT
AAAATTTCTAGCACGAATCAACGTATTAGACAAAATTTGGACCGTATTGTTAAAAGTCAAGCAAATCAGAAAAAATTATC
AGATGCTATTGTAACAGTTAGGAATGAAAGAAACGTTATACCTGTCAAAGCTGAATATCGACAAGATTTTAATGGGATTG
TACATGATCAATCTGCTTCAGGACAAACATTGTATATTGAGCCATCATCAGTTGTTGAAATGAATAATCAAATTAGTCGA
TTACGTCATGATGAAGCAATTGAAAAAGAACGCATTTTAACGCAACTAACTGGTTATGTGGCTGCGGACAAAGATGCACT
GCTTGTGGCAGAACAAGTCATGGGTCAGTTAGATTTTTTAATCGCAAAAGCGAGATATAGTAGAAGTATTAAAGGAACAA
AGCCGATATTTAAAGAGGAACGTACTGTATATTTACCTAAAGCATACCATCCATTATTAAATCGTGAGACTGTTGTAGCT
AATACCATCGAATTTATGGAAGATATTGAAACGGTAATTATTACAGGACCGAATACAGGTGGCAAAACAGTAACATTAAA
AACATTAGGTTTAATTATTGTTATGGCTCAATCAGGATTGTTGATTCCAACACTTGATGGTAGTCAGTTGAGTGTATTTA
AAAATGTATATTGCGATATCGGAGATGAACAATCAATAGAACAATCATTATCAACTTTTTCATCTCATATGACGAATATA
GTTGAGATTTTAAAGCATGCAGACAAACATAGTTTAGTTTTATTTGATGAATTAGGTGCAGGTACAGATCCAAGTGAAGG
TGCTGCTTTAGCAATGAGCATTTTAGACCATGTTAGAAAAATTGGTTCTCTAGTAATGGCAACGACGCACTATCCTGAAC
TTAAAGCATATAGTTATAATCGAGAAGGCGTTATGAATGCGAGTGTAGAATTTGATGTAGATACTTTGAGTCCAACGTAT
AAGTTATTAATGGGTGTACCGGGTCGTTCAAATGCTTTTGACATTTCTAAAAAGTTAGGTCTTAGTTTGAATATTATTAA
TAAGGCTAAGACGATGATTGGTACTGATGAAAAAGAAATAAATGAAATGATTGAATCATTAGAGCGTAATTACAAACGTG
TAGAGACACAGAGGTTAGAACTGGATTGTCTTGTAAAAGAAGCGGAGCAAGTGCATGATGATTTATCTAAGCAGTATCAA
CAATTCCAAAATTATGAAAAGTCTCTAATAGAGGAAGCGAAAGAAAAAGCAAATCAAAAGATTAAAGCTGCAACAAAAGA
AGCCGACGATATTATTAAAGATTTAAGACAATTGCGTGAACAAAAAGGTGCAGATGTTAAAGAACATGAATTGATTGATA
AGAAGAAACGATTAGATGATCATTATGAAGCGAAATCTATAAAGCAAAATGTACAAAAACAAAAATACGATAAAATTGTT
GCTGGTGATGAAGTAAAAGTATTATCTTACGGTCAAAAGGGTGAAGTTTTAGAAATTGTCAATGATGAAGAAGCAATTGT
TCAAATGGGAATTATTAAAATGAAGTTACCTATTGAAGATTTAGAGAAAAAACAAAAAGAAAAAGTTAAGCCAACGAAAA
TGGTTACACGTCAAAATCGTCAAACAATTAAAACTGAACTTGACTTACGAGGCTATCGTTATGAGGATGCTTTAATTGAA
CTAGATCAATATTTAGATCAAGCCGTTTTAAGTAATTACGAACAAGTTTATATCATTCATGGTAAAGGTACAGGTGCACT
TCAAAAAGGTGTACAACAACATTTGAAAAAGCATAAATGTGTTAGTGACTTTAGAGGTGGTATGCCAAGCGAAGGTGGAT
TTGGCGTTACCGTTGCAACTCTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

Smr domain (Smr)

(707-782)

MutS domain V (MutS_V)

(332-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

49.81

100

0.503