Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   I3X02_RS22835 Genome accession   NZ_CP065213
Coordinates   4390473..4392815 (-) Length   780 a.a.
NCBI ID   WP_209367890.1    Uniprot ID   -
Organism   Priestia megaterium strain HGS7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4385473..4397815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3X02_RS22810 - 4385866..4386042 (-) 177 WP_013059305.1 hypothetical protein -
  I3X02_RS22815 - 4386209..4387093 (+) 885 WP_226554880.1 N-acetylmuramoyl-L-alanine amidase -
  I3X02_RS22820 dtd 4387084..4387545 (-) 462 WP_115654948.1 D-aminoacyl-tRNA deacylase -
  I3X02_RS22825 - 4387561..4389759 (-) 2199 WP_014458138.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  I3X02_RS22830 - 4389945..4390457 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  I3X02_RS22835 recJ 4390473..4392815 (-) 2343 WP_209367890.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  I3X02_RS22840 secDF 4392956..4395193 (-) 2238 WP_014458136.1 protein translocase subunit SecDF -
  I3X02_RS22845 - 4395331..4395624 (-) 294 WP_025750585.1 post-transcriptional regulator -
  I3X02_RS22850 spoVB 4395689..4397299 (+) 1611 WP_014458134.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87781.44 Da        Isoelectric Point: 4.7679

>NTDB_id=508161 I3X02_RS22835 WP_209367890.1 4390473..4392815(-) (recJ) [Priestia megaterium strain HGS7]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLQDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDTLNKE
RQQIVNDITEEAIEFVEGMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVGAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=508161 I3X02_RS22835 WP_209367890.1 4390473..4392815(-) (recJ) [Priestia megaterium strain HGS7]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCAAGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCCGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAACT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTCGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTGACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATCGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTCCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATACGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCATTGAATTTGTCGAAGGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCACCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATCACGCTGGAAAACATTGAACAAATGAGTGCGCTGGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAACCATTT
AAAACTCGTATTTGAAGACAGCGGCGTATCGGTTGGTGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATATAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCGTGTATGTTTGCAGGAGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAATGTTTGTTTGTTCAAGGTATCCCTTCTAGGATATACGCTATTTTCCTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAGGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAACAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.754

100

0.559