Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   I2432_RS01815 Genome accession   NZ_CP065054
Coordinates   352141..353076 (+) Length   311 a.a.
NCBI ID   WP_012514940.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain SEZ13     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 353422..394962 352141..353076 flank 346


Gene organization within MGE regions


Location: 352141..394962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2432_RS01815 (LJFMMFNO_00360) amiF 352141..353076 (+) 936 WP_012514940.1 ATP-binding cassette domain-containing protein Regulator
  I2432_RS01820 (LJFMMFNO_00361) rnjA 353706..355388 (-) 1683 WP_012514941.1 ribonuclease J1 -
  I2432_RS01825 (LJFMMFNO_00362) - 355390..355620 (-) 231 WP_014622279.1 DNA-dependent RNA polymerase subunit epsilon -
  I2432_RS01830 (LJFMMFNO_00363) tsaB 355905..356603 (+) 699 WP_206158966.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  I2432_RS01835 (LJFMMFNO_00364) rimI 356606..357031 (+) 426 WP_115250574.1 ribosomal protein S18-alanine N-acetyltransferase -
  I2432_RS01840 (LJFMMFNO_00365) tsaD 357021..358037 (+) 1017 WP_115250575.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  I2432_RS01845 (LJFMMFNO_00366) - 358059..359087 (+) 1029 WP_043030445.1 YdcF family protein -
  I2432_RS01850 (LJFMMFNO_00367) rpsN 359480..359749 (-) 270 WP_012678537.1 30S ribosomal protein S14 -
  I2432_RS01855 (LJFMMFNO_00368) - 359946..361115 (-) 1170 WP_115250576.1 NAD(P)/FAD-dependent oxidoreductase -
  I2432_RS01860 (LJFMMFNO_00369) - 361241..361771 (+) 531 WP_115250577.1 DUF536 domain-containing protein -
  I2432_RS01865 (LJFMMFNO_00370) - 361816..362406 (+) 591 WP_115250578.1 3'-5' exonuclease -
  I2432_RS01870 (LJFMMFNO_00371) - 362497..363219 (+) 723 WP_115250579.1 MerR family transcriptional regulator -
  I2432_RS01875 (LJFMMFNO_00372) - 363261..363560 (-) 300 WP_012678533.1 YbaB/EbfC family nucleoid-associated protein -
  I2432_RS01880 (LJFMMFNO_00373) - 363955..364407 (+) 453 WP_234991596.1 hypothetical protein -
  I2432_RS01885 (LJFMMFNO_00374) - 364547..365233 (+) 687 WP_206158967.1 response regulator transcription factor -
  I2432_RS01890 (LJFMMFNO_00375) - 365235..366278 (+) 1044 WP_115250581.1 HAMP domain-containing sensor histidine kinase -
  I2432_RS01895 (LJFMMFNO_00376) - 366656..368296 (-) 1641 WP_249093326.1 thioester-forming surface-anchored protein -
  I2432_RS01900 (LJFMMFNO_00377) - 368550..370061 (+) 1512 WP_115250583.1 helix-turn-helix domain-containing protein -
  I2432_RS01905 (LJFMMFNO_00378) - 370378..371055 (+) 678 WP_206158969.1 CPBP family glutamic-type intramembrane protease -
  I2432_RS01910 (LJFMMFNO_00379) - 371377..371745 (-) 369 WP_115250585.1 helix-turn-helix transcriptional regulator -
  I2432_RS01915 (LJFMMFNO_00380) - 371924..372145 (+) 222 WP_200831601.1 helix-turn-helix transcriptional regulator -
  I2432_RS01920 (LJFMMFNO_00381) - 372330..372722 (+) 393 WP_115250587.1 pyridoxamine 5'-phosphate oxidase family protein -
  I2432_RS01925 (LJFMMFNO_00382) - 372850..375150 (-) 2301 WP_115250588.1 Xaa-Pro dipeptidyl-peptidase -
  I2432_RS01930 (LJFMMFNO_00383) - 375171..375830 (-) 660 WP_012514975.1 Crp/Fnr family transcriptional regulator -
  I2432_RS01935 (LJFMMFNO_00384) - 375999..377198 (+) 1200 WP_115250589.1 MFS transporter -
  I2432_RS01940 (LJFMMFNO_00385) - 377218..377670 (+) 453 WP_012678520.1 universal stress protein -
  I2432_RS01945 (LJFMMFNO_00386) gla 377771..378619 (+) 849 WP_037580303.1 aquaglyceroporin Gla -
  I2432_RS01950 (LJFMMFNO_00387) - 379109..379861 (+) 753 WP_043038541.1 CppA N-terminal domain-containing protein -
  I2432_RS01955 (LJFMMFNO_00388) - 379858..380799 (+) 942 WP_115250590.1 serine hydrolase domain-containing protein -
  I2432_RS01960 (LJFMMFNO_00389) - 380963..381241 (-) 279 WP_115250591.1 hypothetical protein -
  I2432_RS01965 (LJFMMFNO_00390) pflB 381534..383858 (-) 2325 WP_012678516.1 formate C-acetyltransferase -
  I2432_RS01970 (LJFMMFNO_00391) dinB 384067..385170 (+) 1104 WP_043025192.1 DNA polymerase IV -
  I2432_RS01975 - 385246..386594 (+) 1349 WP_115250806.1 IS3 family transposase -
  I2432_RS01980 (LJFMMFNO_00394) - 386749..387234 (-) 486 WP_115250592.1 hypothetical protein -
  I2432_RS01985 (LJFMMFNO_00395) - 387330..389729 (-) 2400 WP_115251467.1 ATP-dependent RecD-like DNA helicase -
  I2432_RS01990 (LJFMMFNO_00396) lepB 389958..390557 (-) 600 WP_012514985.1 signal peptidase I -
  I2432_RS01995 (LJFMMFNO_00397) rnhC 390582..391490 (-) 909 WP_115250593.1 ribonuclease HIII -
  I2432_RS02000 (LJFMMFNO_00398) - 391608..391919 (+) 312 WP_037580269.1 hypothetical protein -
  I2432_RS02005 (LJFMMFNO_00399) - 391919..392464 (+) 546 WP_200831602.1 CvpA family protein -
  I2432_RS02010 (LJFMMFNO_00400) - 392538..394874 (+) 2337 WP_115250595.1 endonuclease MutS2 -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 35067.17 Da        Isoelectric Point: 8.3565

>NTDB_id=506288 I2432_RS01815 WP_012514940.1 352141..353076(+) (amiF) [Streptococcus equi subsp. zooepidemicus strain SEZ13]
MTEARKKLIEVKNVSLTFNKGKKNEVKAIDRLSFDIYEGEVFGLVGESGSGKTTVGRAILKLYDISDGEIIFNGENVSQL
KGKKLHSFRKHAQMIFQDPQASLNGRMKIRDIVAEGLDIHGLTASKAEREARVQELLKLVGLNQDHLTRYPHEFSGGQRQ
RIGIARALAVKPTFIIADEPISALDVSIQAQVVSLMQQLQKKRGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSDE
VYNHPIHPYTKSLISAIPEPDPEKERRRIAEVYDPSQELDGQERQMYEITPGHFVLATQAEADQYRAERGL

Nucleotide


Download         Length: 936 bp        

>NTDB_id=506288 I2432_RS01815 WP_012514940.1 352141..353076(+) (amiF) [Streptococcus equi subsp. zooepidemicus strain SEZ13]
ATGACAGAAGCTCGTAAAAAATTAATCGAAGTAAAGAATGTTTCCTTAACCTTTAACAAGGGGAAGAAAAACGAGGTTAA
GGCTATTGATCGCCTTAGCTTTGACATTTATGAAGGGGAAGTATTTGGCCTTGTTGGGGAATCAGGCTCAGGCAAGACAA
CAGTTGGTCGAGCTATTTTGAAATTGTATGATATTAGTGATGGCGAGATTATCTTTAATGGAGAAAACGTCTCCCAATTA
AAGGGGAAAAAGCTGCATAGCTTTCGTAAGCATGCGCAAATGATCTTCCAAGATCCTCAGGCTAGTCTGAACGGTCGCAT
GAAAATCAGAGACATTGTTGCAGAGGGCTTAGACATTCATGGCTTGACAGCCTCAAAGGCAGAGCGTGAGGCAAGAGTCC
AAGAGCTGTTAAAGCTTGTTGGGCTTAATCAGGATCATTTGACGCGGTACCCACATGAATTTTCTGGTGGCCAACGCCAG
CGGATTGGCATTGCGCGTGCCCTGGCTGTGAAGCCGACCTTTATTATCGCAGATGAGCCGATTTCAGCGCTTGATGTGTC
GATTCAAGCACAGGTAGTGAGTCTCATGCAGCAATTGCAAAAGAAGCGCGGCTTGACCTATCTCTTTATCGCACACGATT
TGTCTATGGTCAAATACATTTCAGACCGTATTGGTGTGATGCATTGGGGCAAAATGCTTGAGATTGGAACATCTGATGAG
GTTTACAATCACCCGATTCACCCTTATACGAAAAGCTTGATCTCGGCCATTCCTGAGCCGGATCCTGAAAAGGAGCGTAG
GCGCATTGCAGAGGTTTATGATCCTAGTCAAGAGCTAGATGGCCAAGAGCGGCAAATGTATGAAATCACTCCTGGGCATT
TTGTCTTAGCGACGCAGGCCGAAGCCGATCAGTATCGAGCAGAGCGTGGCCTCTAA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(30-181)

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(233-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMD-9

53.922

98.392

0.531
  amiF Streptococcus thermophilus LMG 18311

53.595

98.392

0.527
  amiF Streptococcus salivarius strain HSISS4

53.268

98.392

0.524