Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   I0K03_RS05600 Genome accession   NZ_CP064875
Coordinates   1094703..1098218 (+) Length   1171 a.a.
NCBI ID   WP_200203743.1    Uniprot ID   -
Organism   Bacillus toyonensis strain P18     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1089703..1103218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0K03_RS05565 (I0K03_05565) - 1089756..1090547 (-) 792 WP_000073820.1 phosphotransferase -
  I0K03_RS05570 (I0K03_05570) - 1090567..1091061 (-) 495 WP_000208489.1 DUF6584 family protein -
  I0K03_RS05575 (I0K03_05575) cspA 1091340..1091543 (+) 204 WP_000301522.1 RNA chaperone/antiterminator CspA -
  I0K03_RS05580 (I0K03_05580) - 1091681..1091866 (-) 186 WP_000391370.1 hypothetical protein -
  I0K03_RS05585 (I0K03_05585) - 1092157..1092738 (+) 582 WP_000444438.1 competence protein ComK -
  I0K03_RS05590 (I0K03_05590) - 1093247..1093840 (+) 594 WP_000347508.1 TVP38/TMEM64 family protein -
  I0K03_RS05595 (I0K03_05595) lepB 1094023..1094586 (+) 564 WP_000751914.1 signal peptidase I -
  I0K03_RS05600 (I0K03_05600) addB 1094703..1098218 (+) 3516 WP_200203743.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  I0K03_RS05605 (I0K03_05605) addA 1098215..1101940 (+) 3726 WP_200203745.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  I0K03_RS05610 (I0K03_05610) - 1101964..1102242 (+) 279 WP_200203747.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  I0K03_RS05615 (I0K03_05615) gerPF 1102331..1102546 (-) 216 WP_001141566.1 spore germination protein GerPF -
  I0K03_RS05620 (I0K03_05620) - 1102589..1102975 (-) 387 WP_000902326.1 spore germination protein GerPE -
  I0K03_RS05625 (I0K03_05625) gerPD 1102991..1103185 (-) 195 WP_001052804.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134572.77 Da        Isoelectric Point: 5.0333

>NTDB_id=505536 I0K03_RS05600 WP_200203743.1 1094703..1098218(+) (addB) [Bacillus toyonensis strain P18]
MSLRLVIGRAGSGKSTLCLREVQEELKQRPRGETILYLVPEQMTFQTQQALIESEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLETHYEARPNEKYYDEASVTISTAANL
RAEVEGVAREIRRLVADETYRYRDIAVLLRNGESYYDVMRTLFTDYDIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEMMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDIEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLVANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVIGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTTNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVDGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=505536 I0K03_RS05600 WP_200203743.1 1094703..1098218(+) (addB) [Bacillus toyonensis strain P18]
ATGTCACTTCGATTGGTGATTGGTAGAGCGGGAAGTGGAAAGAGTACACTTTGTTTACGAGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGCGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGAAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCTTGGAAGGTGTTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTGCATATGTTACTTCGTAAAATTGTAGAATCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAGCATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCTGAGCAAAAGTTATTAGCG
AATAAAGTATATGATTTACAGCTATTATATGATGATTTTGAACGTGCTTTAATCGGGAAATATTTAGATTCAGAAGACTA
TTTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAGTATGTAAATGGTGCTGAAATTTATATAGATGGGTTTCATTCAT
TTTCTCCGCAAGAACTTGAAATTGTAAGACAGCTCATGATTTGTGGAGCGAGAGTCACAATTACGTTAACGATAGATGAA
AAAACATTAGCGCAGCCAGTGAATGAACTGGATTTATTTTATGAAACGACGTTAACATATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCTCCGGCATTAGCTCATT
TAGAAACGCATTATGAAGCGCGTCCGAATGAAAAGTATTATGATGAAGCAAGTGTAACAATTAGTACAGCGGCGAATTTA
CGAGCTGAAGTAGAAGGGGTTGCTCGTGAAATTCGTAGACTTGTCGCGGATGAAACGTATCGTTATCGAGATATTGCAGT
ACTTCTTCGTAACGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATGATATTCCACACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCATTAGTAGAGTGTATTCGTTCTGCCTTAGAAATTATTAGCGGAAATTGGCGTTAT
GACGCGGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGGAAAGAAATGATGCGTGAAGAGATGGA
TGAGTTCGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGTT
ACCGTTCACTTGACGATACAAACGGAATGATAACAGATATTGAACGTGAAATGGAAGAGAAAATAAACAGGCTGCGTGAT
GTTGTAAGAACGCCAGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCGGGAACAGTGATGCAAATGTGCGAAGCGGTTTA
CTTATTTTTAGAAGAACTTGACGTTCCAAAGAAGTTAGAAGAATTACGTATACGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCAACAGACCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAAATGCTTGGTGAGGAGAAG
ATGTCACTATCTATGTTCACGGACGTTATGTCGACAGGACTTGAGGCACTTCAATTCGCTAACATTCCGCCATCATTAGA
TCAAGTATTAGTTGCCAATATTGATCACTCCAGGTTATCAGATGTGAAAGCAACGTTTATTATCGGCGTAAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCGGCAGGAATTGAACTC
GCGCCAACGACGAGACAAACTTTATTAGAAGAGCAGTTCGTTATGTATCAGATGGTAACGAGAGCGTCTGAGAAATTATA
TATTTCATGCCCACTTGCAGATGAAGAAGGAAAGACATTACTTGCATCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGACTCGTTTATTACGAATGACGTAAACGACTTATCGCGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTATGTCATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATCTAGATTTTTGGTG
GGACGTCTATAACTTCTACGTCACTTCGGATGAATGGAAACAAAAAAGTAGTCGCGTGTTATCGAGTTTATTCTATCGAA
ATCGTGCGCAGAAACTAAGTACGGTAGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTCTTCAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTAGATGC
ACCAGATATCGGTGAACTATTCCATGCAGCACTGAAGAGAATTGCAGACAGGCTATTACGCGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAAAGACAAATTTTA
TTAAGCTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
CGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAGGTGCCATTCGGTATGGGCGGTACGGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTATCGGCCGTATTGACCGTGTGGATAAGGCTGAAGATGAAAACGGA
ACATTCCTTCGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTCGTTACTACAAATGCACAAACGTGGATGAAAAAAGGCCACGCAGCATCACCTGCTGGTG
TACTGTACTTTCATATTCATAACCCGATTGTTGAAATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGCCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGCATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTTATTGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCATGTACGTTCTGTAACTTCAAATCTGTCTGTCAGTTTGATGAATCACTTGAAGATAA
TCAATTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGACGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494