Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   IMX31_RS02750 Genome accession   NZ_CP064793
Coordinates   521766..524108 (+) Length   780 a.a.
NCBI ID   WP_195781810.1    Uniprot ID   -
Organism   Priestia megaterium strain IGA-FME-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 516766..529108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMX31_RS02735 spoVB 517282..518892 (-) 1611 WP_229754520.1 stage V sporulation protein B -
  IMX31_RS02740 - 518957..519250 (+) 294 WP_028411692.1 post-transcriptional regulator -
  IMX31_RS02745 secDF 519388..521625 (+) 2238 WP_195781809.1 protein translocase subunit SecDF -
  IMX31_RS02750 recJ 521766..524108 (+) 2343 WP_195781810.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  IMX31_RS02755 - 524124..524636 (+) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  IMX31_RS02760 - 524822..527020 (+) 2199 WP_116073761.1 RelA/SpoT family protein -
  IMX31_RS02765 dtd 527036..527497 (+) 462 WP_013059307.1 D-aminoacyl-tRNA deacylase -
  IMX31_RS02770 - 527488..528372 (-) 885 WP_229754521.1 N-acetylmuramoyl-L-alanine amidase -
  IMX31_RS02775 - 528539..528715 (+) 177 WP_013059305.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87874.47 Da        Isoelectric Point: 4.6980

>NTDB_id=504781 IMX31_RS02750 WP_195781810.1 521766..524108(+) (recJ) [Priestia megaterium strain IGA-FME-1]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDVQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDTLNKE
RQQIVNDITEEAVEFVEGMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASDVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKILIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACMLAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSTKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFERSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=504781 IMX31_RS02750 WP_195781810.1 521766..524108(+) (recJ) [Priestia megaterium strain IGA-FME-1]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
TACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGCACTTCCGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GTGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCTGTCGAAGAAACTGCGTATGCACGTGAATTA
AATTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGTAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAGCG
TGAAGAAATTAATGAAGATACAATTGGATTTGCGATGGCTCCTCGTATTAATGCAGTAGGACGTCTTCAAGATGCAGACC
CAGCTGTTCATTTGCTTATGACCACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATACGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTTGAAGGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAAGGAGAAGGCTGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCGACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTAGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAATTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTCGTGTTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCGGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGTATGCTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAATGTTTGTTTGTCCAAGGTATCCCTTCTAGAATATACGCTATTTTCTTGACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTACGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTGAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)

DHHA1 domain (DHHA1)

(305-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.499

100

0.556