Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KQ220_RS18975 Genome accession   NZ_CP076709
Coordinates   3917360..3918124 (+) Length   254 a.a.
NCBI ID   WP_001136197.1    Uniprot ID   -
Organism   Escherichia coli O158:H23 strain CFIAFB20140388     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3912360..3923124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ220_RS18955 (KQ220_18955) acpT 3913314..3913901 (+) 588 WP_112005954.1 4'-phosphopantetheinyl transferase AcpT -
  KQ220_RS18960 (KQ220_18960) nikA 3914012..3915586 (+) 1575 WP_112005956.1 nickel ABC transporter substrate-binding protein -
  KQ220_RS18965 (KQ220_18965) nikB 3915586..3916530 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KQ220_RS18970 (KQ220_18970) nikC 3916527..3917360 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  KQ220_RS18975 (KQ220_18975) amiE 3917360..3918124 (+) 765 WP_001136197.1 nickel import ATP-binding protein NikD Regulator
  KQ220_RS18980 (KQ220_18980) nikE 3918121..3918927 (+) 807 WP_048971478.1 nickel import ATP-binding protein NikE -
  KQ220_RS18985 (KQ220_18985) nikR 3918933..3919334 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KQ220_RS18990 (KQ220_18990) - 3919454..3919813 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  KQ220_RS18995 (KQ220_18995) - 3919810..3920085 (-) 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin -
  KQ220_RS19000 (KQ220_19000) yhhJ 3920158..3921282 (-) 1125 WP_001318088.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26813.43 Da        Isoelectric Point: 6.6886

>NTDB_id=504333 KQ220_RS18975 WP_001136197.1 3917360..3918124(+) (amiE) [Escherichia coli O158:H23 strain CFIAFB20140388]
MPQQIELRDIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=504333 KQ220_RS18975 WP_001136197.1 3917360..3918124(+) (amiE) [Escherichia coli O158:H23 strain CFIAFB20140388]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTAGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGACAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGTGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGTTGGAAAACGCCGCGCGCGTGT
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402