Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KQ221_RS01235 Genome accession   NZ_CP076704
Coordinates   271045..271809 (-) Length   254 a.a.
NCBI ID   WP_097446329.1    Uniprot ID   -
Organism   Escherichia coli O8:H28 strain BMH-17-0027     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 266045..276809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ221_RS01210 (KQ221_01210) yhhJ 267902..269026 (+) 1125 WP_001314210.1 ABC transporter permease -
  KQ221_RS01215 (KQ221_01215) - 269130..269360 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  KQ221_RS01220 (KQ221_01220) - 269357..269716 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  KQ221_RS01225 (KQ221_01225) nikR 269836..270237 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KQ221_RS01230 (KQ221_01230) nikE 270242..271048 (-) 807 WP_097446330.1 nickel import ATP-binding protein NikE -
  KQ221_RS01235 (KQ221_01235) amiE 271045..271809 (-) 765 WP_097446329.1 nickel import ATP-binding protein NikD Regulator
  KQ221_RS01240 (KQ221_01240) nikC 271809..272642 (-) 834 WP_097446328.1 nickel ABC transporter permease subunit NikC -
  KQ221_RS01245 (KQ221_01245) nikB 272639..273583 (-) 945 WP_088144577.1 nickel ABC transporter permease subunit NikB -
  KQ221_RS01250 (KQ221_01250) nikA 273583..275157 (-) 1575 WP_097446327.1 nickel ABC transporter substrate-binding protein -
  KQ221_RS01255 (KQ221_01255) acpT 275268..275855 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26791.29 Da        Isoelectric Point: 5.8663

>NTDB_id=504117 KQ221_RS01235 WP_097446329.1 271045..271809(-) (amiE) [Escherichia coli O8:H28 strain BMH-17-0027]
MPQQIELRNIALEAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPQHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=504117 KQ221_RS01235 WP_097446329.1 271045..271809(-) (amiE) [Escherichia coli O8:H28 strain BMH-17-0027]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTAGAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAGATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACACACCATGCACACTCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGTTATACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCTGTGCTGTGTGAATCGCCG
TTTATCATCGCCGATGAGCCGACCACCGACCTTGACGTGGTAGCGCAGGCGCGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTGGTGGCGCGTCTGGCGGATGACGTGGCGGTAA
TGTCTGACGGTAAAATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCTCAACATGCGGTGACGCGAAGCCTG
GTTTCAGCGCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398