Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KP725_RS01215 Genome accession   NZ_CP076645
Coordinates   267188..267952 (-) Length   254 a.a.
NCBI ID   WP_047081515.1    Uniprot ID   -
Organism   Escherichia coli strain MSI001     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 262188..272952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP725_RS01205 (KP725_01205) nikR 265978..266379 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  KP725_RS01210 (KP725_01210) nikE 266385..267191 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  KP725_RS01215 (KP725_01215) amiE 267188..267952 (-) 765 WP_047081515.1 nickel import ATP-binding protein NikD Regulator
  KP725_RS01220 (KP725_01220) nikC 267952..268785 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  KP725_RS01225 (KP725_01225) nikB 268782..269726 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  KP725_RS01230 (KP725_01230) nikA 269726..271300 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  KP725_RS01235 (KP725_01235) acpT 271411..271998 (-) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26849.46 Da        Isoelectric Point: 6.5992

>NTDB_id=503291 KP725_RS01215 WP_047081515.1 267188..267952(-) (amiE) [Escherichia coli strain MSI001]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKLVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=503291 KP725_RS01215 WP_047081515.1 267188..267952(-) (amiE) [Escherichia coli strain MSI001]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACTGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398