Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HWI43_RS06250 Genome accession   NZ_CP064528
Coordinates   1307388..1308455 (+) Length   355 a.a.
NCBI ID   WP_408020299.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain UMCG370     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1302388..1313455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWI43_RS06220 (HWI43_06165) - 1302806..1304266 (+) 1461 WP_145334441.1 rhomboid family intramembrane serine protease -
  HWI43_RS06225 (HWI43_06170) - 1304250..1304450 (+) 201 WP_002446537.1 YqgQ family protein -
  HWI43_RS06230 (HWI43_06175) - 1304450..1305436 (+) 987 WP_002446536.1 ROK family glucokinase -
  HWI43_RS06235 (HWI43_06180) - 1305436..1305762 (+) 327 WP_001831234.1 MTH1187 family thiamine-binding protein -
  HWI43_RS06240 (HWI43_06185) - 1305762..1306385 (+) 624 WP_002446535.1 MBL fold metallo-hydrolase -
  HWI43_RS06245 (HWI43_06190) comGA 1306442..1307416 (+) 975 WP_002446534.1 competence type IV pilus ATPase ComGA Machinery gene
  HWI43_RS06250 (HWI43_06195) comGB 1307388..1308455 (+) 1068 WP_408020299.1 competence type IV pilus assembly protein ComGB Machinery gene
  HWI43_RS06255 (HWI43_06200) comGC 1308473..1308790 (+) 318 WP_002446532.1 competence type IV pilus major pilin ComGC Machinery gene
  HWI43_RS06260 (HWI43_06205) comGD 1308780..1309217 (+) 438 WP_002446531.1 competence type IV pilus minor pilin ComGD -
  HWI43_RS06265 (HWI43_06210) - 1309204..1309497 (+) 294 WP_002446530.1 hypothetical protein -
  HWI43_RS06270 (HWI43_06215) - 1309421..1309912 (+) 492 WP_002457724.1 competence type IV pilus minor pilin ComGF -
  HWI43_RS06275 (HWI43_06220) - 1310077..1310589 (+) 513 WP_408020300.1 shikimate kinase -
  HWI43_RS06280 (HWI43_06225) gcvT 1310807..1311898 (+) 1092 WP_002457722.1 glycine cleavage system aminomethyltransferase GcvT -
  HWI43_RS06285 (HWI43_06230) gcvPA 1311918..1313264 (+) 1347 WP_002446526.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 42384.58 Da        Isoelectric Point: 10.0598

>NTDB_id=502753 HWI43_RS06250 WP_408020299.1 1307388..1308455(+) (comGB) [Staphylococcus epidermidis strain UMCG370]
MKKLSINTFKYKRNKYLTEIQSINLLQRLHQLLSHGFTLYQSFKFLNSYFKYKERTINEKIIQHLQNGATCYDILKMIGY
PELVLLQIKFAENYGNIDEALVDTVQYMKRNLKAKKRLIKTLQYPVALISIFLFILTILNITVIPQFQQLYETMNVKLST
FQNLLTLIITLLPKLTFIFIFMSGIAFFITYKFYYYLPIERKLKSILKIPMINTYYKIYRTYQLSNQLSLFYRNGTSLQQ
IVQIYRNEQDNEFLKFLGNCLFKEANRGLPLPTILMNLQCFQNDLIKFIEQGEKNGKFDIELKLYSQMLLQQFEEKVLKQ
TKFIQPIIFFILGIFIVSLYLVIMLPMFELMQTIK

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=502753 HWI43_RS06250 WP_408020299.1 1307388..1308455(+) (comGB) [Staphylococcus epidermidis strain UMCG370]
GTGAAGAAGTTGTCGATAAATACATTTAAATATAAGAGGAATAAATATCTTACTGAAATACAATCAATAAACTTATTACA
GAGATTACATCAGCTTTTAAGTCACGGATTCACCTTATATCAAAGTTTTAAATTTTTAAACTCTTATTTTAAATATAAAG
AACGAACAATAAATGAAAAGATTATCCAACATCTACAAAACGGCGCTACATGTTATGATATTTTAAAAATGATAGGGTAT
CCAGAATTAGTTCTTCTTCAAATAAAATTTGCTGAAAACTACGGTAACATTGATGAGGCTCTCGTTGATACTGTTCAATA
TATGAAAAGAAATTTGAAAGCTAAAAAACGACTCATCAAAACCTTACAATATCCTGTTGCATTAATTTCTATCTTCTTAT
TCATATTAACCATTTTAAATATAACTGTCATACCTCAATTTCAACAACTTTATGAGACTATGAATGTTAAATTATCAACA
TTTCAAAATCTACTAACTCTTATTATTACCCTTCTTCCCAAGCTAACTTTCATTTTTATCTTTATGAGTGGTATAGCATT
TTTTATCACTTATAAATTTTACTATTATCTACCAATTGAGAGAAAATTAAAATCTATTTTAAAAATCCCAATGATTAATA
CGTATTATAAAATATATAGAACTTATCAACTTTCCAATCAGCTTTCTTTATTTTACAGAAATGGTACAAGTCTTCAACAA
ATTGTCCAAATATATCGTAATGAGCAAGATAACGAATTTCTTAAATTTCTGGGTAATTGTCTTTTTAAAGAAGCTAATAG
AGGGCTCCCGTTACCTACTATATTAATGAATCTACAATGTTTTCAAAATGATTTAATTAAATTCATAGAACAAGGAGAGA
AAAATGGAAAATTTGATATAGAATTAAAATTATACAGTCAAATGTTATTACAGCAATTTGAGGAAAAAGTGTTAAAACAA
ACAAAATTTATACAACCTATCATCTTCTTTATCTTGGGAATTTTTATTGTATCTTTATACTTAGTCATTATGCTTCCTAT
GTTCGAACTTATGCAAACAATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-145)

Type II secretion system (T2SS), protein F (T2SSF)

(225-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

48.315

100

0.485
  comGB Staphylococcus aureus N315

48.315

100

0.485