Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   A7A15_RS18530 Genome accession   NZ_CP076282
Coordinates   3776266..3777030 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain 442/2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3771266..3782030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7A15_RS18510 (A7A15_18450) acpT 3772220..3772807 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  A7A15_RS18515 (A7A15_18455) nikA 3772918..3774492 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  A7A15_RS18520 (A7A15_18460) nikB 3774492..3775436 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  A7A15_RS18525 (A7A15_18465) nikC 3775433..3776266 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  A7A15_RS18530 (A7A15_18470) amiE 3776266..3777030 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  A7A15_RS18535 (A7A15_18475) nikE 3777027..3777833 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  A7A15_RS18540 (A7A15_18480) nikR 3777839..3778240 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  A7A15_RS18545 (A7A15_18485) - 3778439..3779185 (+) 747 WP_000839066.1 GntR family transcriptional regulator -
  A7A15_RS18550 (A7A15_18490) - 3779210..3779683 (+) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  A7A15_RS18555 (A7A15_18495) - 3779680..3779961 (+) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  A7A15_RS18560 (A7A15_18500) - 3780038..3781396 (+) 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=501845 A7A15_RS18530 WP_001136236.1 3776266..3777030(+) (amiE) [Escherichia coli strain 442/2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=501845 A7A15_RS18530 WP_001136236.1 3776266..3777030(+) (amiE) [Escherichia coli strain 442/2]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398