Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   IQ863_RS20290 Genome accession   NZ_CP064270
Coordinates   3934380..3936722 (+) Length   780 a.a.
NCBI ID   WP_014458137.1    Uniprot ID   A0A8D3WXX7
Organism   Priestia megaterium strain N447-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3929380..3941722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQ863_RS20275 spoVB 3929896..3931506 (-) 1611 WP_243555939.1 stage V sporulation protein B -
  IQ863_RS20280 - 3931571..3931864 (+) 294 WP_025750585.1 post-transcriptional regulator -
  IQ863_RS20285 secDF 3932002..3934239 (+) 2238 WP_014458136.1 protein translocase subunit SecDF -
  IQ863_RS20290 recJ 3934380..3936722 (+) 2343 WP_014458137.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  IQ863_RS20295 - 3936738..3937250 (+) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  IQ863_RS20300 - 3937436..3939634 (+) 2199 WP_014458138.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  IQ863_RS20305 dtd 3939650..3940111 (+) 462 WP_014458139.1 D-aminoacyl-tRNA deacylase -
  IQ863_RS20310 - 3940102..3940986 (-) 885 WP_226554880.1 N-acetylmuramoyl-L-alanine amidase -
  IQ863_RS20315 - 3941153..3941329 (+) 177 WP_013059305.1 hypothetical protein -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87839.47 Da        Isoelectric Point: 4.7430

>NTDB_id=500473 IQ863_RS20290 WP_014458137.1 3934380..3936722(+) (recJ) [Priestia megaterium strain N447-3]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLQDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDTLNKE
RQQIVNDITEEAIEFVEGMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLECLFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=500473 IQ863_RS20290 WP_014458137.1 3934380..3936722(+) (recJ) [Priestia megaterium strain N447-3]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCAAGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCCGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAATTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAACT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTCGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTGACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATCGGATTTGCGATGGCTCCTCGCATTAATGCAGTAGGACGTCTCCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATACGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCATTGAATTTGTCGAAGGTATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCACCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAGCTGCGCAGACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATCACGCTGGAAAACATTGAACAAATGAGTGCGCTGGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAACCATTT
AAAACTCGTATTTGAAGACAGCGGCGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTATTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATATAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCGTGTATGTTTGCAGGAGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAG
AAGTGGCTTGAATGTTTGTTTGTTCAAGGTATCCCTTCTAGGATATACGCTATTTTCCTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAGGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAACAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.882

100

0.56