Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   IRJ21_RS17985 Genome accession   NZ_CP064091
Coordinates   3657874..3661374 (+) Length   1166 a.a.
NCBI ID   WP_029973173.1    Uniprot ID   -
Organism   Bacillus velezensis strain C1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3652874..3666374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ21_RS17960 - 3653034..3654191 (-) 1158 WP_021494949.1 AbrB family transcriptional regulator -
  IRJ21_RS17965 - 3654358..3655554 (-) 1197 WP_014417364.1 MFS transporter -
  IRJ21_RS17970 - 3655570..3656190 (-) 621 WP_165882194.1 TetR/AcrR family transcriptional regulator -
  IRJ21_RS17975 - 3656442..3656951 (-) 510 WP_007610501.1 ferritin-like domain-containing protein -
  IRJ21_RS17980 - 3657020..3657733 (-) 714 WP_165882205.1 DUF421 domain-containing protein -
  IRJ21_RS17985 addB 3657874..3661374 (+) 3501 WP_029973173.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  IRJ21_RS17990 addA 3661361..3665065 (+) 3705 WP_173613628.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  IRJ21_RS17995 sbcD 3665130..3666302 (+) 1173 WP_029973171.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133306.83 Da        Isoelectric Point: 5.3571

>NTDB_id=499096 IRJ21_RS17985 WP_029973173.1 3657874..3661374(+) (addB) [Bacillus velezensis strain C1]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHRHVPELACIESQYDVRPAAAYTGGQEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTSGERFVYRRFVSLDEDFAQTDQEIEMEQMLNETKEW
MAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEEAGNMIEAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKESDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRVV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIVNAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFQSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=499096 IRJ21_RS17985 WP_029973173.1 3657874..3661374(+) (addB) [Bacillus velezensis strain C1]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGAGTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTATGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCGGCATGTGCCGGAGCTTGCCTGTATAG
AGTCGCAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAACTACTGTATCGCCTACGGCATTAAAGGGGAGCGCTGGACGAGCGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACAGATCAGGAAATAGAGATGGAGCAAATGCTGAATGAGACGAAAGAGTGG
ATGGCCGCGCCCCTTGTCCGGCTTCAAAACCGGATGAAAAAGGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGAAAAAGGCAGCGGGCCGAAGAGGCCGGAAACATGATCGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCGCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGGGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCGGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGACCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGTCTGATCACCGGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCCCATTTTGCATCACACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGACGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAGTCGTT
GATTATAAATCAAGTGACAGGGGGCTTGATCTGGCGGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATATCTTGA
CTTATCGATTACACATTCCGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTTCATATTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACGTTGGAGGAAGGGCGTTCAAATATCGTCAATGCCGGTTT
GAAAAAGGACGGTTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACAGGATTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTCAATCGGTATGCCAGTTTGATGAGTCGCTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAGAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.276

100

0.833