Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IGI33_RS02030 Genome accession   NZ_CP075477
Coordinates   422566..423033 (-) Length   155 a.a.
NCBI ID   WP_025653356.1    Uniprot ID   -
Organism   Escherichia coli strain NT1W25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417566..428033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI33_RS02005 rpsG 417707..418177 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  IGI33_RS02010 fusA 418274..420388 (+) 2115 WP_000124700.1 elongation factor G -
  IGI33_RS02015 tuf 420459..421643 (+) 1185 WP_000031783.1 elongation factor Tu -
  IGI33_RS02020 bfd 421826..422020 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  IGI33_RS02025 bfr 422093..422569 (+) 477 WP_000675515.1 bacterioferritin -
  IGI33_RS02030 pilD 422566..423033 (-) 468 WP_025653356.1 prepilin peptidase Machinery gene
  IGI33_RS02035 rpsJ 423412..423723 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  IGI33_RS02040 rplC 423756..424385 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  IGI33_RS02045 rplD 424396..425001 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  IGI33_RS02050 rplW 424998..425300 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  IGI33_RS02055 rplB 425318..426139 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  IGI33_RS02060 rpsS 426156..426434 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  IGI33_RS02065 rplV 426449..426781 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  IGI33_RS02070 rpsC 426799..427500 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  IGI33_RS02075 rplP 427513..427923 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16921.05 Da        Isoelectric Point: 8.1296

>NTDB_id=498353 IGI33_RS02030 WP_025653356.1 422566..423033(-) (pilD) [Escherichia coli strain NT1W25]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWAFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=498353 IGI33_RS02030 WP_025653356.1 422566..423033(-) (pilD) [Escherichia coli strain NT1W25]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGGCCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361