Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   IGI24_RS01850 Genome accession   NZ_CP075472
Coordinates   267539..268303 (-) Length   254 a.a.
NCBI ID   WP_001136249.1    Uniprot ID   -
Organism   Escherichia coli strain NT1F25     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 262539..273303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI24_RS01840 nikR 266329..266730 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  IGI24_RS01845 nikE 266736..267542 (-) 807 WP_000173663.1 nickel import ATP-binding protein NikE -
  IGI24_RS01850 amiE 267539..268303 (-) 765 WP_001136249.1 nickel import ATP-binding protein NikD Regulator
  IGI24_RS01855 nikC 268303..269136 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  IGI24_RS01860 nikB 269133..270077 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  IGI24_RS01865 nikA 270077..271651 (-) 1575 WP_032298533.1 nickel ABC transporter substrate-binding protein -
  IGI24_RS01870 acpT 271762..272349 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26831.45 Da        Isoelectric Point: 6.5992

>NTDB_id=498189 IGI24_RS01850 WP_001136249.1 267539..268303(-) (amiE) [Escherichia coli strain NT1F25]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAVALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=498189 IGI24_RS01850 WP_001136249.1 267539..268303(-) (amiE) [Escherichia coli strain NT1F25]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGTGGCGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398