Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   IGI31_RS01145 Genome accession   NZ_CP075465
Coordinates   255361..256125 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain NT1N34     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 250361..261125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI31_RS01135 nikR 254151..254552 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  IGI31_RS01140 nikE 254558..255364 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  IGI31_RS01145 amiE 255361..256125 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  IGI31_RS01150 nikC 256125..256958 (-) 834 WP_121851340.1 nickel ABC transporter permease subunit NikC -
  IGI31_RS01155 nikB 256955..257899 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  IGI31_RS01160 nikA 257899..259473 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  IGI31_RS01165 acpT 259584..260171 (-) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=498030 IGI31_RS01145 WP_001136236.1 255361..256125(-) (amiE) [Escherichia coli strain NT1N34]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=498030 IGI31_RS01145 WP_001136236.1 255361..256125(-) (amiE) [Escherichia coli strain NT1N34]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398