Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   H4F95_RS24650 Genome accession   NZ_CP075061
Coordinates   4459130..4459894 (+) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain TCM_B_2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4454130..4464894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4F95_RS24630 (H4F95_24640) acpT 4455084..4455671 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  H4F95_RS24635 (H4F95_24645) nikA 4455782..4457356 (+) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  H4F95_RS24640 (H4F95_24650) nikB 4457356..4458300 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  H4F95_RS24645 (H4F95_24655) nikC 4458297..4459130 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  H4F95_RS24650 (H4F95_24660) amiE 4459130..4459894 (+) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  H4F95_RS24655 (H4F95_24665) nikE 4459891..4460697 (+) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  H4F95_RS24660 (H4F95_24670) nikR 4460703..4461104 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  H4F95_RS24665 (H4F95_24675) - 4461307..4461564 (+) 258 WP_213206464.1 hypothetical protein -
  H4F95_RS24670 (H4F95_24680) - 4462248..4462487 (+) 240 WP_213206477.1 hypothetical protein -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=497262 H4F95_RS24650 WP_001136236.1 4459130..4459894(+) (amiE) [Escherichia coli strain TCM_B_2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=497262 H4F95_RS24650 WP_001136236.1 4459130..4459894(+) (amiE) [Escherichia coli strain TCM_B_2]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398