Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIL87_RS02465 Genome accession   NZ_CP074998
Coordinates   537277..537744 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 6883     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532277..542744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL87_RS02440 (KIL87_02430) rpsG 532418..532888 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIL87_RS02445 (KIL87_02435) fusA 532985..535099 (+) 2115 WP_000124700.1 elongation factor G -
  KIL87_RS02450 (KIL87_02440) tuf 535170..536354 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIL87_RS02455 (KIL87_02445) bfd 536537..536731 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIL87_RS02460 (KIL87_02450) bfr 536804..537280 (+) 477 WP_000675515.1 bacterioferritin -
  KIL87_RS02465 (KIL87_02455) pilD 537277..537744 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIL87_RS02470 (KIL87_02460) rpsJ 538123..538434 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIL87_RS02475 (KIL87_02465) rplC 538467..539096 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIL87_RS02480 (KIL87_02470) rplD 539107..539712 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIL87_RS02485 (KIL87_02475) rplW 539709..540011 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIL87_RS02490 (KIL87_02480) rplB 540029..540850 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIL87_RS02495 (KIL87_02485) rpsS 540867..541145 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIL87_RS02500 (KIL87_02490) rplV 541160..541492 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIL87_RS02505 (KIL87_02495) rpsC 541510..542211 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIL87_RS02510 (KIL87_02500) rplP 542224..542634 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=496958 KIL87_RS02465 WP_000339836.1 537277..537744(-) (pilD) [Escherichia coli strain 6883]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=496958 KIL87_RS02465 WP_000339836.1 537277..537744(-) (pilD) [Escherichia coli strain 6883]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361