Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIL89_RS27130 Genome accession   NZ_CP074991
Coordinates   5239315..5239782 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 10159     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5234315..5244782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL89_RS27085 (KIL89_26745) rplP 5234425..5234835 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIL89_RS27090 (KIL89_26750) rpsC 5234848..5235549 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIL89_RS27095 (KIL89_26755) rplV 5235567..5235899 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIL89_RS27100 (KIL89_26760) rpsS 5235914..5236192 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIL89_RS27105 (KIL89_26765) rplB 5236209..5237030 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIL89_RS27110 (KIL89_26770) rplW 5237048..5237350 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIL89_RS27115 (KIL89_26775) rplD 5237347..5237952 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIL89_RS27120 (KIL89_26780) rplC 5237963..5238592 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIL89_RS27125 (KIL89_26785) rpsJ 5238625..5238936 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIL89_RS27130 (KIL89_26790) pilD 5239315..5239782 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIL89_RS27135 (KIL89_26795) bfr 5239779..5240255 (-) 477 WP_000675515.1 bacterioferritin -
  KIL89_RS27140 (KIL89_26800) bfd 5240328..5240522 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIL89_RS27145 (KIL89_26805) tuf 5240705..5241889 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIL89_RS27150 (KIL89_26810) fusA 5241960..5244074 (-) 2115 WP_000124700.1 elongation factor G -
  KIL89_RS27155 (KIL89_26815) rpsG 5244171..5244641 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=496849 KIL89_RS27130 WP_000339836.1 5239315..5239782(+) (pilD) [Escherichia coli strain 10159]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=496849 KIL89_RS27130 WP_000339836.1 5239315..5239782(+) (pilD) [Escherichia coli strain 10159]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361