Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HGR01_RS18545 Genome accession   NZ_CP063785
Coordinates   4489403..4490509 (-) Length   368 a.a.
NCBI ID   WP_045874646.1    Uniprot ID   -
Organism   Tolypothrix sp. PCC 7712     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4484403..4495509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGR01_RS18525 (HGR01_17955) - 4484528..4484971 (-) 444 WP_045874650.1 pyridoxamine 5'-phosphate oxidase family protein -
  HGR01_RS18530 (HGR01_17960) - 4485172..4487616 (-) 2445 WP_045874649.1 type IV pilus secretin family protein -
  HGR01_RS18535 (HGR01_17965) - 4487837..4488610 (-) 774 WP_045874648.1 pilus assembly protein PilO -
  HGR01_RS18540 (HGR01_17970) - 4488607..4489398 (-) 792 WP_045874647.1 PilN domain-containing protein -
  HGR01_RS18545 (HGR01_17975) pilM 4489403..4490509 (-) 1107 WP_045874646.1 type IV pilus assembly protein PilM Machinery gene
  HGR01_RS18550 (HGR01_17980) - 4490651..4491952 (+) 1302 WP_045874645.1 ABC transporter substrate-binding protein -
  HGR01_RS18555 (HGR01_17985) - 4492339..4493274 (-) 936 WP_045874644.1 aldo/keto reductase -
  HGR01_RS18560 (HGR01_17990) - 4493854..4495134 (+) 1281 WP_045874643.1 S41 family peptidase -
  HGR01_RS18565 (HGR01_17995) - 4495171..4495347 (+) 177 WP_168161054.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40529.56 Da        Isoelectric Point: 4.4017

>NTDB_id=496799 HGR01_RS18545 WP_045874646.1 4489403..4490509(-) (pilM) [Tolypothrix sp. PCC 7712]
MVKSFNSLFSKSNKGVGIELAPERVNIVQLRKQRQGLKVEALTSIPVPEGVMMDGQIADAPAMAQLIQQGLAESKIKASR
VATGVTGRDSIVRLIPVPAELDDKELREMVLNHEAALYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKEVTEAY
INTFEQAGLQIDILEINSFAMIRTIREQLRQFSPQEAAVLVDIEFDSSEIAIIVNGVPQFSRTVPIGTYQMQYALSKAMS
LPASRDMEMLYGMTIMTTNADGGKTGLTDINPGMAALLRVLGELTDELRRSIDFYLNQSENLEVAQIFLAGPGGGLQQLD
EFFTQRLSLPTAQIDPITSLSIEVSEEKYPAVQRAGLATVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=496799 HGR01_RS18545 WP_045874646.1 4489403..4490509(-) (pilM) [Tolypothrix sp. PCC 7712]
GTGGTGAAAAGCTTCAATAGTCTGTTTAGCAAATCTAATAAAGGGGTCGGTATTGAACTAGCACCAGAAAGGGTGAATAT
AGTTCAATTACGCAAACAACGTCAAGGCTTAAAAGTAGAAGCCTTAACATCAATACCAGTTCCCGAAGGTGTAATGATGG
ACGGTCAAATCGCTGATGCGCCAGCGATGGCGCAGTTAATTCAGCAGGGTTTGGCTGAAAGCAAAATTAAAGCTTCTCGG
GTTGCCACTGGGGTCACAGGACGAGATTCAATTGTCCGTTTAATACCTGTACCAGCAGAGTTAGATGATAAAGAACTGCG
AGAAATGGTGCTAAACCATGAAGCAGCGTTGTATTTACCCTATCCGCGTGAAGAAGCTGATGTCGATTATCAAAAACTTG
GGTACTTTGTAGATGAAGATGGTATTGAAAAAGTACAAGTACTTTTAGTAGCTACACGTAAGGAGGTAACGGAAGCATAT
ATTAATACATTTGAGCAGGCTGGATTGCAAATCGATATTTTAGAAATCAACAGTTTTGCCATGATTCGGACAATCCGCGA
GCAATTGCGGCAATTCAGCCCCCAAGAAGCAGCAGTATTAGTTGATATCGAATTTGATAGTTCAGAAATTGCCATTATTG
TCAACGGAGTGCCGCAATTTTCGCGTACTGTACCTATCGGTACTTATCAAATGCAATATGCCCTCTCAAAGGCTATGAGT
CTACCTGCAAGCCGAGATATGGAAATGTTGTACGGCATGACGATTATGACAACAAACGCCGATGGCGGAAAAACTGGGCT
AACCGATATTAATCCAGGTATGGCAGCTTTATTAAGAGTTTTGGGAGAACTCACCGATGAACTGCGTCGTTCTATCGATT
TTTACCTCAATCAAAGTGAAAATTTGGAGGTAGCACAAATTTTTCTAGCAGGCCCAGGCGGTGGCTTGCAACAGCTAGAT
GAGTTTTTCACCCAAAGATTAAGCTTACCAACTGCACAAATCGATCCGATAACATCTTTGTCCATAGAAGTTAGTGAGGA
AAAATACCCTGCAGTACAACGCGCTGGTTTAGCGACAGTACTGGGTTTAGGAATGCGGGAGGTATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(283-367)

Type IV pilus assembly protein PilM; (PilM_2)

(17-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.395

99.728

0.652