Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIL96_RS02270 Genome accession   NZ_CP074961
Coordinates   489726..490193 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 01_3327     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484726..495193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIL96_RS02245 (KIL96_02240) rpsG 484867..485337 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIL96_RS02250 (KIL96_02245) fusA 485434..487548 (+) 2115 WP_000124700.1 elongation factor G -
  KIL96_RS02255 (KIL96_02250) tuf 487619..488803 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIL96_RS02260 (KIL96_02255) bfd 488986..489180 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIL96_RS02265 (KIL96_02260) bfr 489253..489729 (+) 477 WP_000675515.1 bacterioferritin -
  KIL96_RS02270 (KIL96_02265) pilD 489726..490193 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIL96_RS02275 (KIL96_02270) rpsJ 490572..490883 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIL96_RS02280 (KIL96_02275) rplC 490916..491545 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIL96_RS02285 (KIL96_02280) rplD 491556..492161 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIL96_RS02290 (KIL96_02285) rplW 492158..492460 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIL96_RS02295 (KIL96_02290) rplB 492478..493299 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIL96_RS02300 (KIL96_02295) rpsS 493316..493594 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIL96_RS02305 (KIL96_02300) rplV 493609..493941 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIL96_RS02310 (KIL96_02305) rpsC 493959..494660 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIL96_RS02315 (KIL96_02310) rplP 494673..495083 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=496227 KIL96_RS02270 WP_000339836.1 489726..490193(-) (pilD) [Escherichia coli strain 01_3327]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=496227 KIL96_RS02270 WP_000339836.1 489726..490193(-) (pilD) [Escherichia coli strain 01_3327]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361