Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM00_RS02200 Genome accession   NZ_CP074946
Coordinates   475193..475660 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 05_3471     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470193..480660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM00_RS02175 (KIM00_02170) rpsG 470334..470804 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIM00_RS02180 (KIM00_02175) fusA 470901..473015 (+) 2115 WP_000124700.1 elongation factor G -
  KIM00_RS02185 (KIM00_02180) tuf 473086..474270 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIM00_RS02190 (KIM00_02185) bfd 474453..474647 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM00_RS02195 (KIM00_02190) bfr 474720..475196 (+) 477 WP_000675515.1 bacterioferritin -
  KIM00_RS02200 (KIM00_02195) pilD 475193..475660 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM00_RS02205 (KIM00_02200) rpsJ 476039..476350 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM00_RS02210 (KIM00_02205) rplC 476383..477012 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM00_RS02215 (KIM00_02210) rplD 477023..477628 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM00_RS02220 (KIM00_02215) rplW 477625..477927 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM00_RS02225 (KIM00_02220) rplB 477945..478766 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM00_RS02230 (KIM00_02225) rpsS 478783..479061 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM00_RS02235 (KIM00_02230) rplV 479076..479408 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM00_RS02240 (KIM00_02235) rpsC 479426..480127 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM00_RS02245 (KIM00_02240) rplP 480140..480550 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=495903 KIM00_RS02200 WP_000339836.1 475193..475660(-) (pilD) [Escherichia coli strain 05_3471]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=495903 KIM00_RS02200 WP_000339836.1 475193..475660(-) (pilD) [Escherichia coli strain 05_3471]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361