Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM14_RS25310 Genome accession   NZ_CP074895
Coordinates   4968832..4969299 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 2009C_3689     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4963832..4974299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM14_RS25265 (KIM14_24965) rplP 4963942..4964352 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIM14_RS25270 (KIM14_24970) rpsC 4964365..4965066 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM14_RS25275 (KIM14_24975) rplV 4965084..4965416 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM14_RS25280 (KIM14_24980) rpsS 4965431..4965709 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM14_RS25285 (KIM14_24985) rplB 4965726..4966547 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM14_RS25290 (KIM14_24990) rplW 4966565..4966867 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM14_RS25295 (KIM14_24995) rplD 4966864..4967469 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM14_RS25300 (KIM14_25000) rplC 4967480..4968109 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM14_RS25305 (KIM14_25005) rpsJ 4968142..4968453 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM14_RS25310 (KIM14_25010) pilD 4968832..4969299 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM14_RS25315 (KIM14_25015) bfr 4969296..4969772 (-) 477 WP_000675515.1 bacterioferritin -
  KIM14_RS25320 (KIM14_25020) bfd 4969845..4970039 (-) 195 WP_032346861.1 bacterioferritin-associated ferredoxin -
  KIM14_RS25325 (KIM14_25025) tuf 4970222..4971406 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIM14_RS25330 (KIM14_25030) fusA 4971477..4973591 (-) 2115 WP_000124700.1 elongation factor G -
  KIM14_RS25335 (KIM14_25035) rpsG 4973688..4974158 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=494837 KIM14_RS25310 WP_000339836.1 4968832..4969299(+) (pilD) [Escherichia coli strain 2009C_3689]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=494837 KIM14_RS25310 WP_000339836.1 4968832..4969299(+) (pilD) [Escherichia coli strain 2009C_3689]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361