Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM24_RS08520 Genome accession   NZ_CP074859
Coordinates   1663067..1663534 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 2013C_3964     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1658067..1668534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM24_RS08475 (KIM24_08430) rplP 1658177..1658587 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIM24_RS08480 (KIM24_08435) rpsC 1658600..1659301 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM24_RS08485 (KIM24_08440) rplV 1659319..1659651 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM24_RS08490 (KIM24_08445) rpsS 1659666..1659944 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM24_RS08495 (KIM24_08450) rplB 1659961..1660782 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM24_RS08500 (KIM24_08455) rplW 1660800..1661102 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM24_RS08505 (KIM24_08460) rplD 1661099..1661704 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM24_RS08510 (KIM24_08465) rplC 1661715..1662344 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM24_RS08515 (KIM24_08470) rpsJ 1662377..1662688 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM24_RS08520 (KIM24_08475) pilD 1663067..1663534 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM24_RS08525 (KIM24_08480) bfr 1663531..1664007 (-) 477 WP_000675515.1 bacterioferritin -
  KIM24_RS08530 (KIM24_08485) bfd 1664080..1664274 (-) 195 WP_032346861.1 bacterioferritin-associated ferredoxin -
  KIM24_RS08535 (KIM24_08490) tuf 1664457..1665641 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIM24_RS08540 (KIM24_08495) fusA 1665712..1667826 (-) 2115 WP_000124700.1 elongation factor G -
  KIM24_RS08545 (KIM24_08500) rpsG 1667923..1668393 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=493928 KIM24_RS08520 WP_000339836.1 1663067..1663534(+) (pilD) [Escherichia coli strain 2013C_3964]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=493928 KIM24_RS08520 WP_000339836.1 1663067..1663534(+) (pilD) [Escherichia coli strain 2013C_3964]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361