Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM26_RS02480 Genome accession   NZ_CP074853
Coordinates   527621..528088 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 7_11_88A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522621..533088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM26_RS02455 (KIM26_02435) rpsG 522762..523232 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIM26_RS02460 (KIM26_02440) fusA 523329..525443 (+) 2115 WP_000124700.1 elongation factor G -
  KIM26_RS02465 (KIM26_02445) tuf 525514..526698 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIM26_RS02470 (KIM26_02450) bfd 526881..527075 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM26_RS02475 (KIM26_02455) bfr 527148..527624 (+) 477 WP_000675517.1 bacterioferritin -
  KIM26_RS02480 (KIM26_02460) pilD 527621..528088 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM26_RS02485 (KIM26_02465) rpsJ 528467..528778 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM26_RS02490 (KIM26_02470) rplC 528811..529440 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM26_RS02495 (KIM26_02475) rplD 529451..530056 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM26_RS02500 (KIM26_02480) rplW 530053..530355 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM26_RS02505 (KIM26_02485) rplB 530373..531194 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM26_RS02510 (KIM26_02490) rpsS 531211..531489 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM26_RS02515 (KIM26_02495) rplV 531504..531836 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM26_RS02520 (KIM26_02500) rpsC 531854..532555 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM26_RS02525 (KIM26_02505) rplP 532568..532978 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=493756 KIM26_RS02480 WP_000339836.1 527621..528088(-) (pilD) [Escherichia coli strain 7_11_88A]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=493756 KIM26_RS02480 WP_000339836.1 527621..528088(-) (pilD) [Escherichia coli strain 7_11_88A]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361