Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KIM28_RS28105 Genome accession   NZ_CP074845
Coordinates   5415335..5416099 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 7_12_30A     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5410335..5421099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM28_RS28085 (KIM28_27730) acpT 5411289..5411876 (+) 588 WP_000285771.1 4'-phosphopantetheinyl transferase AcpT -
  KIM28_RS28090 (KIM28_27735) nikA 5411987..5413561 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  KIM28_RS28095 (KIM28_27740) nikB 5413561..5414505 (+) 945 WP_000947060.1 nickel ABC transporter permease subunit NikB -
  KIM28_RS28100 (KIM28_27745) nikC 5414502..5415335 (+) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  KIM28_RS28105 (KIM28_27750) amiE 5415335..5416099 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  KIM28_RS28110 (KIM28_27755) nikE 5416096..5416902 (+) 807 WP_000173630.1 nickel import ATP-binding protein NikE -
  KIM28_RS28115 (KIM28_27760) nikR 5416908..5417309 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=493653 KIM28_RS28105 WP_001136229.1 5415335..5416099(+) (amiE) [Escherichia coli strain 7_12_30A]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=493653 KIM28_RS28105 WP_001136229.1 5415335..5416099(+) (amiE) [Escherichia coli strain 7_12_30A]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398