Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   KIM32_RS27625 Genome accession   NZ_CP074826
Coordinates   5342157..5342921 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain 7_26_9A     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 5337157..5347921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM32_RS27605 (KIM32_27290) acpT 5338111..5338698 (+) 588 WP_000285771.1 4'-phosphopantetheinyl transferase AcpT -
  KIM32_RS27610 (KIM32_27295) nikA 5338809..5340383 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  KIM32_RS27615 (KIM32_27300) nikB 5340383..5341327 (+) 945 WP_000947060.1 nickel ABC transporter permease subunit NikB -
  KIM32_RS27620 (KIM32_27305) nikC 5341324..5342157 (+) 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
  KIM32_RS27625 (KIM32_27310) amiE 5342157..5342921 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  KIM32_RS27630 (KIM32_27315) nikE 5342918..5343724 (+) 807 WP_000173630.1 nickel import ATP-binding protein NikE -
  KIM32_RS27635 (KIM32_27320) nikR 5343730..5344131 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=493335 KIM32_RS27625 WP_001136229.1 5342157..5342921(+) (amiE) [Escherichia coli strain 7_26_9A]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=493335 KIM32_RS27625 WP_001136229.1 5342157..5342921(+) (amiE) [Escherichia coli strain 7_26_9A]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398