Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM32_RS26610 Genome accession   NZ_CP074826
Coordinates   5152780..5153247 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 7_26_9A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5147780..5158247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM32_RS26565 (KIM32_26250) rplP 5147890..5148300 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIM32_RS26570 (KIM32_26255) rpsC 5148313..5149014 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM32_RS26575 (KIM32_26260) rplV 5149032..5149364 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM32_RS26580 (KIM32_26265) rpsS 5149379..5149657 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM32_RS26585 (KIM32_26270) rplB 5149674..5150495 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM32_RS26590 (KIM32_26275) rplW 5150513..5150815 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM32_RS26595 (KIM32_26280) rplD 5150812..5151417 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM32_RS26600 (KIM32_26285) rplC 5151428..5152057 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM32_RS26605 (KIM32_26290) rpsJ 5152090..5152401 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM32_RS26610 (KIM32_26295) pilD 5152780..5153247 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM32_RS26615 (KIM32_26300) bfr 5153244..5153720 (-) 477 WP_000675515.1 bacterioferritin -
  KIM32_RS26620 (KIM32_26305) bfd 5153793..5153987 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM32_RS26625 (KIM32_26310) tuf 5154170..5155354 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIM32_RS26630 (KIM32_26315) fusA 5155425..5157539 (-) 2115 WP_000124700.1 elongation factor G -
  KIM32_RS26635 (KIM32_26320) rpsG 5157636..5158106 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=493330 KIM32_RS26610 WP_000339836.1 5152780..5153247(+) (pilD) [Escherichia coli strain 7_26_9A]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=493330 KIM32_RS26610 WP_000339836.1 5152780..5153247(+) (pilD) [Escherichia coli strain 7_26_9A]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361