Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM39_RS25690 Genome accession   NZ_CP074791
Coordinates   4997906..4998373 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 94_0962     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4992906..5003373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM39_RS25645 (KIM39_25310) rplP 4993016..4993426 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIM39_RS25650 (KIM39_25315) rpsC 4993439..4994140 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM39_RS25655 (KIM39_25320) rplV 4994158..4994490 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM39_RS25660 (KIM39_25325) rpsS 4994505..4994783 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM39_RS25665 (KIM39_25330) rplB 4994800..4995621 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM39_RS25670 (KIM39_25335) rplW 4995639..4995941 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM39_RS25675 (KIM39_25340) rplD 4995938..4996543 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM39_RS25680 (KIM39_25345) rplC 4996554..4997183 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM39_RS25685 (KIM39_25350) rpsJ 4997216..4997527 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM39_RS25690 (KIM39_25355) pilD 4997906..4998373 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM39_RS25695 (KIM39_25360) bfr 4998370..4998846 (-) 477 WP_000675515.1 bacterioferritin -
  KIM39_RS25700 (KIM39_25365) bfd 4998919..4999113 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM39_RS25705 (KIM39_25370) tuf 4999296..5000480 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIM39_RS25710 (KIM39_25375) fusA 5000551..5002665 (-) 2115 WP_000124700.1 elongation factor G -
  KIM39_RS25715 (KIM39_25380) rpsG 5002762..5003232 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=492769 KIM39_RS25690 WP_000339836.1 4997906..4998373(+) (pilD) [Escherichia coli strain 94_0962]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=492769 KIM39_RS25690 WP_000339836.1 4997906..4998373(+) (pilD) [Escherichia coli strain 94_0962]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361