Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM43_RS02480 Genome accession   NZ_CP074773
Coordinates   527623..528090 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 97_3250     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522623..533090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM43_RS02455 (KIM43_02435) rpsG 522764..523234 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIM43_RS02460 (KIM43_02440) fusA 523331..525445 (+) 2115 WP_000124700.1 elongation factor G -
  KIM43_RS02465 (KIM43_02445) tuf 525516..526700 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIM43_RS02470 (KIM43_02450) bfd 526883..527077 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM43_RS02475 (KIM43_02455) bfr 527150..527626 (+) 477 WP_000675517.1 bacterioferritin -
  KIM43_RS02480 (KIM43_02460) pilD 527623..528090 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM43_RS02485 (KIM43_02465) rpsJ 528469..528780 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM43_RS02490 (KIM43_02470) rplC 528813..529442 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM43_RS02495 (KIM43_02475) rplD 529453..530058 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM43_RS02500 (KIM43_02480) rplW 530055..530357 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM43_RS02505 (KIM43_02485) rplB 530375..531196 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM43_RS02510 (KIM43_02490) rpsS 531213..531491 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM43_RS02515 (KIM43_02495) rplV 531506..531838 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM43_RS02520 (KIM43_02500) rpsC 531856..532557 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM43_RS02525 (KIM43_02505) rplP 532570..532980 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=492389 KIM43_RS02480 WP_000339836.1 527623..528090(-) (pilD) [Escherichia coli strain 97_3250]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=492389 KIM43_RS02480 WP_000339836.1 527623..528090(-) (pilD) [Escherichia coli strain 97_3250]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361