Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM44_RS25460 Genome accession   NZ_CP074769
Coordinates   4985026..4985493 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain 99_0288     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4980026..4990493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM44_RS25415 (KIM44_25095) rplP 4980136..4980546 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KIM44_RS25420 (KIM44_25100) rpsC 4980559..4981260 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM44_RS25425 (KIM44_25105) rplV 4981278..4981610 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM44_RS25430 (KIM44_25110) rpsS 4981625..4981903 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM44_RS25435 (KIM44_25115) rplB 4981920..4982741 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM44_RS25440 (KIM44_25120) rplW 4982759..4983061 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM44_RS25445 (KIM44_25125) rplD 4983058..4983663 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM44_RS25450 (KIM44_25130) rplC 4983674..4984303 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM44_RS25455 (KIM44_25135) rpsJ 4984336..4984647 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM44_RS25460 (KIM44_25140) pilD 4985026..4985493 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM44_RS25465 (KIM44_25145) bfr 4985490..4985966 (-) 477 WP_000675515.1 bacterioferritin -
  KIM44_RS25470 (KIM44_25150) bfd 4986039..4986233 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM44_RS25475 (KIM44_25155) tuf 4986416..4987600 (-) 1185 WP_000031783.1 elongation factor Tu -
  KIM44_RS25480 (KIM44_25160) fusA 4987671..4989785 (-) 2115 WP_000124700.1 elongation factor G -
  KIM44_RS25485 (KIM44_25165) rpsG 4989882..4990352 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=492361 KIM44_RS25460 WP_000339836.1 4985026..4985493(+) (pilD) [Escherichia coli strain 99_0288]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=492361 KIM44_RS25460 WP_000339836.1 4985026..4985493(+) (pilD) [Escherichia coli strain 99_0288]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361