Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   IL989_RS05200 Genome accession   NZ_CP062984
Coordinates   1027929..1031429 (+) Length   1166 a.a.
NCBI ID   WP_193351548.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain PP19 isolate PP19     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1022929..1036429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL989_RS05175 (IL989_05175) - 1023094..1024251 (-) 1158 WP_193351545.1 AbrB family transcriptional regulator -
  IL989_RS05180 (IL989_05180) - 1024417..1025613 (-) 1197 WP_088612550.1 MFS transporter -
  IL989_RS05185 (IL989_05185) - 1025629..1026249 (-) 621 WP_125122346.1 TetR/AcrR family transcriptional regulator -
  IL989_RS05190 (IL989_05190) - 1026479..1027006 (-) 528 WP_193351546.1 ferritin-like domain-containing protein -
  IL989_RS05195 (IL989_05195) - 1027075..1027788 (-) 714 WP_193351547.1 DUF421 domain-containing protein -
  IL989_RS05200 (IL989_05200) addB 1027929..1031429 (+) 3501 WP_193351548.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  IL989_RS05205 (IL989_05205) addA 1031416..1035120 (+) 3705 WP_193351908.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  IL989_RS05210 (IL989_05210) sbcD 1035187..1036359 (+) 1173 WP_071347113.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 133744.38 Da        Isoelectric Point: 5.3596

>NTDB_id=492231 IL989_RS05200 WP_193351548.1 1027929..1031429(+) (addB) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
MGVEFLVGRSGSGKTRLIIDSIQEELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHDFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERILSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADIIKGARVYVDGFYQFTPQELRVLEQLIMHAEHITFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHVPELACIESQYDVRPAAVYTGGQEAFTVIQAQNR
RAELEGIAREIQSLVRDRGYRYRDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTNGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
MAEPLIRLQNRLKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQQAEEAGNMIEAQQHGQAWDAVIQLLEEFTGMMGEDEM
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKEPDHERAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CRFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYDAVERLAPRLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMELDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSERDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEANDDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=492231 IL989_RS05200 WP_193351548.1 1027929..1031429(+) (addB) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGAAGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGCTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCACACGGGG
GGCATGAACCGTCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAACTGATTGAAGAACATAAGCACGATTT
TAAAGTCTATCAAAAAGCGAGTGACAAAACCGGATTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAACCTGAAGACGTCCGCCGCATGGCAGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATACTGTCTGAA
AAACTTCATGACTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGCCACTATTTGCATTCAGAGGATTATTT
GACACTGCTCGCTCAGCAGATTCCTTTGGCGGATATTATAAAAGGCGCCCGTGTTTATGTCGACGGCTTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTCGAGCAGCTGATCATGCATGCTGAACATATCACATTCTCTTTGACGGCGGACAGCCCT
TCGGCGGAACAGGCTCCGGATGAGCTCGACCTGTTCAGAATGACCGGCAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAGTTGAATGCCGATATTTCCTGCAAGGAATTGCATGGAACGAAGCGGCATCAGCATGTGCCGGAGCTTGCCTGTATAG
AATCGCAGTACGATGTGCGTCCCGCGGCTGTGTATACCGGCGGACAGGAAGCTTTTACCGTCATACAGGCACAGAACAGA
AGAGCTGAACTCGAGGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACAGAGGATACCGGTACAGAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGACTTGCTGAAGGAAGTGTTTGCAGATTACGGGCTTCCTTATTTTATAGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGCTCGAGCTTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCCCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTTGA
TCAGCTTGAAAACTACTGTATCGCCTACGGAATCAAAGGCGAGCGCTGGACGAACGGGGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAATGAGACGAGAGAGTGG
ATGGCCGAGCCGCTCATCCGGCTTCAAAACCGGCTGAAAAAAGCGAAAACGGTTCAAAGCATGGCAGAAGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGACAGCAGGCCGAAGAGGCCGGAAACATGATTGAAG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAAGAGTTTACGGGCATGATGGGTGAAGATGAAATG
TCTTTGGCGCTGTTCCAGCAAATGCTTGAAACGGGAACCGAATCGCTTCATTTTTCTTTAATTCCTCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGATCTGTCCAGAATGTACGGCACATCCTGCACATTTGTCATCGGAGCCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGAGAGTGGCTGAAAGCGGTCGGAGTGGAGCTTTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCAAGTCCGTCTGACCGTCTGTATGT
GACATATCCGATTGCCGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACAGGCTCGGCGAGCTGTTTCCCG
ATCACCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAACAGCTTCAGTATCTCGTCAATAAACAG
GTGGCGCAGACGTATACGGCAAGCCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTGTGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGCCTGATCACGAGCGTGCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGAAATGAGGCAAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCACATTAAAGGAAGCGTCTCTCGTATGGAGGCGTTTAATGCC
TGCCGGTTTTCTCATTTTGCATCTCACGGGCTGCAATTAAAAGAACGGCAGTTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAGTGCCGGAATTTTTCCTATGATGCGGTGGAACGGCTTGCGCCAAGGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGGCACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTCTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTTGTCCCGATCGGCCTTGAACTCGGTTTTGGCGGAAGCGGGCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGCCGGATCGACCGCGTGGATAAAGCGGAAAGCTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGATAAGGGGCTTGATCTCGCTGAGGTATATTACGGGCTCGCTCTGCAAATGCTCACATACCTTGA
CTTATCAATTACACACTCAGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATTCATGATC
CGATGATTCAGGCCAGTCTGCCGATGGAACTTGATGAAATCGAGCAGGAAATTTTTAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGCCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATTATCAATGCCGGTTT
GAAAAAGGACGGATCTTTACGGTCCGATTCAGCGGCGGTCAGCGAACGGGATTTTCATCTGCTGACAGACCATGTGAGGC
GGACATTTGAGCAGGCGGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAATCGGTGTGCCAGTTTGATGAGTCGCTTAAAGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACTATACTGGATTGGCTGAAAAAGGAGGCGAATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(307-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

83.19

100

0.832