Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KIM46_RS02195 Genome accession   NZ_CP074764
Coordinates   474857..475324 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain B1_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469857..480324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM46_RS02170 (KIM46_02165) rpsG 469998..470468 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KIM46_RS02175 (KIM46_02170) fusA 470565..472679 (+) 2115 WP_000124700.1 elongation factor G -
  KIM46_RS02180 (KIM46_02175) tuf 472750..473934 (+) 1185 WP_000031783.1 elongation factor Tu -
  KIM46_RS02185 (KIM46_02180) bfd 474117..474311 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KIM46_RS02190 (KIM46_02185) bfr 474384..474860 (+) 477 WP_000675515.1 bacterioferritin -
  KIM46_RS02195 (KIM46_02190) pilD 474857..475324 (-) 468 WP_000339836.1 prepilin peptidase Machinery gene
  KIM46_RS02200 (KIM46_02195) rpsJ 475703..476014 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KIM46_RS02205 (KIM46_02200) rplC 476047..476676 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KIM46_RS02210 (KIM46_02205) rplD 476687..477292 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KIM46_RS02215 (KIM46_02210) rplW 477289..477591 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KIM46_RS02220 (KIM46_02215) rplB 477609..478430 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KIM46_RS02225 (KIM46_02220) rpsS 478447..478725 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KIM46_RS02230 (KIM46_02225) rplV 478740..479072 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KIM46_RS02235 (KIM46_02230) rpsC 479090..479791 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KIM46_RS02240 (KIM46_02235) rplP 479804..480214 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=492147 KIM46_RS02195 WP_000339836.1 474857..475324(-) (pilD) [Escherichia coli strain B1_2]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=492147 KIM46_RS02195 WP_000339836.1 474857..475324(-) (pilD) [Escherichia coli strain B1_2]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361