Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU85_RS02050 Genome accession   NZ_CP074052
Coordinates   456984..457451 (-) Length   155 a.a.
NCBI ID   WP_022646190.1    Uniprot ID   -
Organism   Escherichia coli strain PD23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451984..462451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU85_RS02025 (KFU85_01990) rpsG 452125..452595 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KFU85_RS02030 (KFU85_01995) fusA 452692..454806 (+) 2115 WP_000124700.1 elongation factor G -
  KFU85_RS02035 (KFU85_02000) tuf 454877..456061 (+) 1185 WP_000031783.1 elongation factor Tu -
  KFU85_RS02040 (KFU85_02005) bfd 456244..456438 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KFU85_RS02045 (KFU85_02010) bfr 456511..456987 (+) 477 WP_000675515.1 bacterioferritin -
  KFU85_RS02050 (KFU85_02015) pilD 456984..457451 (-) 468 WP_022646190.1 A24 family peptidase Machinery gene
  KFU85_RS02055 (KFU85_02020) rpsJ 457830..458141 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KFU85_RS02060 (KFU85_02025) rplC 458174..458803 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KFU85_RS02065 (KFU85_02030) rplD 458814..459419 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KFU85_RS02070 (KFU85_02035) rplW 459416..459718 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KFU85_RS02075 (KFU85_02040) rplB 459736..460557 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KFU85_RS02080 (KFU85_02045) rpsS 460574..460852 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KFU85_RS02085 (KFU85_02050) rplV 460867..461199 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KFU85_RS02090 (KFU85_02055) rpsC 461217..461918 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KFU85_RS02095 (KFU85_02060) rplP 461931..462341 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17064.20 Da        Isoelectric Point: 8.1255

>NTDB_id=490496 KFU85_RS02050 WP_022646190.1 456984..457451(-) (pilD) [Escherichia coli strain PD23]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLATAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=490496 KFU85_RS02050 WP_022646190.1 456984..457451(-) (pilD) [Escherichia coli strain PD23]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATTCTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGACTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368