Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU86_RS12335 Genome accession   NZ_CP073996
Coordinates   2514969..2515436 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain PM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2509969..2520436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU86_RS12290 (KFU86_12240) rplP 2510079..2510489 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  KFU86_RS12295 (KFU86_12245) rpsC 2510502..2511203 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  KFU86_RS12300 (KFU86_12250) rplV 2511221..2511553 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  KFU86_RS12305 (KFU86_12255) rpsS 2511568..2511846 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  KFU86_RS12310 (KFU86_12260) rplB 2511863..2512684 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  KFU86_RS12315 (KFU86_12265) rplW 2512702..2513004 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  KFU86_RS12320 (KFU86_12270) rplD 2513001..2513606 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  KFU86_RS12325 (KFU86_12275) rplC 2513617..2514246 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  KFU86_RS12330 (KFU86_12280) rpsJ 2514279..2514590 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  KFU86_RS12335 (KFU86_12285) pilD 2514969..2515436 (+) 468 WP_000339836.1 A24 family peptidase Machinery gene
  KFU86_RS12340 (KFU86_12290) bfr 2515433..2515909 (-) 477 WP_000675515.1 bacterioferritin -
  KFU86_RS12345 (KFU86_12295) bfd 2515982..2516176 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KFU86_RS12350 (KFU86_12300) tuf 2516359..2517543 (-) 1185 WP_000031783.1 elongation factor Tu -
  KFU86_RS12355 (KFU86_12305) fusA 2517614..2519728 (-) 2115 WP_000124700.1 elongation factor G -
  KFU86_RS12360 (KFU86_12310) rpsG 2519825..2520295 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=489570 KFU86_RS12335 WP_000339836.1 2514969..2515436(+) (pilD) [Escherichia coli strain PM7]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=489570 KFU86_RS12335 WP_000339836.1 2514969..2515436(+) (pilD) [Escherichia coli strain PM7]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361