Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   KEF49_RS13145 Genome accession   NZ_CP073635
Coordinates   2611400..2613757 (-) Length   785 a.a.
NCBI ID   WP_212098614.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2606400..2618757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS13120 (KEF49_13120) - 2606903..2607682 (-) 780 WP_212098605.1 enoyl-CoA hydratase -
  KEF49_RS13125 (KEF49_13125) - 2607696..2608280 (-) 585 WP_014470798.1 TetR/AcrR family transcriptional regulator -
  KEF49_RS13130 (KEF49_13130) - 2608369..2610057 (-) 1689 WP_212098608.1 AMP-binding protein -
  KEF49_RS13135 (KEF49_13135) - 2610163..2610840 (+) 678 WP_212098611.1 DUF2711 family protein -
  KEF49_RS13140 (KEF49_13140) - 2610980..2611384 (-) 405 WP_003152541.1 DUF350 domain-containing protein -
  KEF49_RS13145 (KEF49_13145) mutS/mutS2 2611400..2613757 (-) 2358 WP_212098614.1 endonuclease MutS2 Machinery gene
  KEF49_RS13150 (KEF49_13150) polX 2613778..2615490 (-) 1713 WP_212098617.1 DNA polymerase/3'-5' exonuclease PolX -
  KEF49_RS13155 (KEF49_13155) - 2615596..2616129 (-) 534 WP_212098620.1 CvpA family protein -
  KEF49_RS13160 (KEF49_13160) zapA 2616137..2616394 (-) 258 WP_003152533.1 cell division protein ZapA -
  KEF49_RS13165 (KEF49_13165) rnhC 2616528..2617469 (+) 942 WP_212098624.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87343.69 Da        Isoelectric Point: 6.3218

>NTDB_id=487665 KEF49_RS13145 WP_212098614.1 2611400..2613757(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain Ba13]
MQQKVLSSLEFHKVKEQITAHAASSLGREKLLQLKPLTDLADIQKQLDEVEEASAIMRLRGHAPFGGLTDIRSALRRAEI
GSVLTPAEFTELSGLLYAVKQMKHFISQMTEDGVSIPLIQAHAEELITLGDLEREINSCIDDHGEVLDHASPALRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRMLTEHTAEHTQEIVQNVEVLQTLDFIFAKARYAKAMKATKPFMNGDGFIRLKKARHPLLPQDQVVAN
DIELGGDYTTIVITGPNTGGKTVTLKTLGLLTIMAQAGLHIPADEGSEAAVFDHVFADIGDEQSIEQSLSTFSSHMVNIV
SILKDVSENSLVLFDELGAGTDPQEGAALAMSILDEVHRTNARVLATTHYPELKAYGYNRQGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISSRLGLPEHIIGQAKSEMTAEHNEVDLMIASLEKSKKRADEELSETESIRKEAEKLHKELQQQIIE
LNAQKDKLMEEAEQKAAEKLEDAAKEAEQIIRELRSIKQEHRSFKEHELIDAKKRLGDAMPAFEKSKQPERKTEKKRELK
PGDEVKVLTFGQKGALLEKTGEKEWNVQIGILKMKVKEKDLEFLKSAPEPKKEKAITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTIVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=487665 KEF49_RS13145 WP_212098614.1 2611400..2613757(-) (mutS/mutS2) [Bacillus amyloliquefaciens strain Ba13]
GTGCAGCAAAAAGTGTTATCATCACTTGAATTTCATAAAGTAAAAGAACAGATTACCGCACATGCCGCCTCATCGCTCGG
CCGGGAAAAGCTTCTGCAGCTGAAGCCTTTGACTGATCTCGCCGACATCCAAAAACAGCTGGATGAAGTCGAAGAAGCAT
CCGCCATCATGCGCTTAAGAGGCCATGCTCCGTTCGGCGGTCTGACGGATATAAGATCGGCTTTGCGGCGGGCGGAAATC
GGCAGCGTGCTGACCCCGGCGGAATTCACCGAATTGTCCGGGCTGCTGTATGCCGTTAAGCAAATGAAGCACTTTATCAG
CCAAATGACAGAAGACGGCGTCAGCATTCCGCTGATACAGGCCCATGCAGAAGAGCTGATTACATTGGGCGATTTGGAAA
GGGAGATTAATTCCTGCATTGATGATCACGGCGAAGTGCTTGATCATGCATCTCCCGCTTTAAGAGGAATCCGCACGCAG
CTCAGAACGCTTGAATCAAGAGTGAGAGACCGGCTTGAATCGATGCTGCGTTCATCTTCCGCTTCAAAAATGCTGTCTGA
CACGATCGTTACGATTCGGAATGACCGCTTTGTCATTCCGGTCAAGCAGGAATACAGATCAAGCTACGGCGGGATCGTGC
ATGATACTTCTTCATCAGGTGCGACGCTGTTTATTGAACCGCAGGCGATCGTCGATATGAACAACTCGCTTCAGCAGGCG
AAGGTGAAAGAAAAACAGGAAATCGAACGGATTCTCCGGATGCTGACGGAGCATACGGCTGAACACACACAAGAGATTGT
TCAAAATGTGGAAGTGCTGCAGACACTGGATTTCATTTTCGCAAAAGCGAGATACGCAAAAGCGATGAAGGCGACAAAAC
CGTTCATGAACGGAGACGGCTTTATCCGTCTGAAAAAAGCGCGCCATCCGCTGCTGCCTCAAGACCAAGTCGTGGCCAAC
GATATTGAACTGGGCGGAGATTATACGACGATCGTCATCACGGGTCCGAATACGGGCGGTAAAACCGTTACGTTAAAAAC
GCTCGGGCTGTTGACGATAATGGCGCAGGCAGGACTGCACATACCGGCTGATGAAGGATCGGAAGCCGCCGTATTTGATC
ATGTTTTTGCCGATATCGGTGATGAACAGTCGATCGAACAAAGCCTGAGTACGTTCTCATCTCACATGGTCAATATCGTC
AGTATTTTAAAAGACGTGTCCGAAAACAGTCTGGTGCTGTTTGATGAGCTCGGTGCCGGAACCGACCCGCAGGAAGGGGC
GGCGCTTGCCATGAGCATTCTTGACGAAGTCCACCGGACAAACGCCAGAGTGCTTGCGACAACTCATTATCCGGAGCTGA
AAGCATACGGTTATAACAGACAAGGCGTCATGAATGCAAGCGTTGAATTTGATATTGAAACGCTTTCGCCGACCTACAAG
CTCCTGATCGGAGTGCCGGGCCGAAGCAACGCTTTTGAAATCTCAAGCCGCCTCGGCCTGCCGGAGCATATCATCGGCCA
GGCGAAGTCAGAAATGACCGCCGAACACAATGAAGTCGATCTGATGATCGCGTCACTTGAAAAAAGTAAAAAAAGAGCGG
ATGAAGAGCTTTCTGAAACCGAATCAATCAGAAAAGAAGCAGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATTGAG
CTGAATGCCCAGAAAGACAAACTGATGGAAGAAGCCGAACAAAAAGCAGCGGAAAAACTGGAAGACGCCGCAAAAGAAGC
GGAACAGATCATTCGCGAGCTCCGGTCCATCAAGCAAGAACACAGATCCTTTAAGGAACACGAGCTGATTGATGCCAAAA
AACGTCTCGGAGACGCGATGCCGGCTTTTGAAAAATCAAAGCAGCCGGAAAGAAAAACAGAGAAAAAACGCGAGCTGAAG
CCGGGTGACGAAGTAAAGGTGCTCACATTCGGACAAAAAGGGGCGCTGCTTGAAAAAACCGGCGAGAAAGAATGGAATGT
CCAAATCGGCATCCTTAAAATGAAGGTGAAGGAAAAAGATCTTGAGTTTCTCAAGTCCGCTCCTGAACCGAAAAAAGAAA
AAGCCATCACCGCCGTAAAAGGGAAGGATTATCACGTGTCCCTTGAACTTGATCTGCGGGGAGAGCGTTACGAAAATGCC
CTCAGCCGCGTCGAAAAATATTTAGATGACGCGGTGCTTGCCGGCTATCCGAGAGTGTCCATCATTCACGGAAAAGGCAC
GGGCGCCCTCCGAAAAGGGGTGCAGGATCTTCTGAAAAACCACCGCAGCGTGAAAAGCTCGCGGTTCGGGGAAGCGGGAG
AAGGAGGATCAGGCGTAACGATTGTCGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

87.643

100

0.876