Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IF561_RS15080 Genome accession   NZ_CP062150
Coordinates   3196330..3197346 (+) Length   338 a.a.
NCBI ID   WP_100662586.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain 19-VB00998     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3191330..3202346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF561_RS15065 (IF561_15055) - 3191754..3192482 (-) 729 WP_015297397.1 redoxin family protein -
  IF561_RS15070 (IF561_15060) oxyR 3192653..3193561 (+) 909 WP_025636038.1 DNA-binding transcriptional regulator OxyR -
  IF561_RS15075 (IF561_15065) - 3193617..3196187 (-) 2571 WP_264400992.1 penicillin-binding protein 1A -
  IF561_RS15080 (IF561_15070) pilM 3196330..3197346 (+) 1017 WP_100662586.1 type IV pilus biogenesis protein PilM Machinery gene
  IF561_RS15085 (IF561_15075) pilN 3197330..3197908 (+) 579 WP_264400993.1 PilN domain-containing protein Machinery gene
  IF561_RS15090 (IF561_15080) pilO 3197901..3198494 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  IF561_RS15095 (IF561_15085) pilP 3198484..3198999 (+) 516 WP_161608959.1 pilus assembly protein PilP Machinery gene
  IF561_RS15100 (IF561_15090) pilQ 3199031..3200779 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  IF561_RS15105 (IF561_15095) aroK 3200968..3201486 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37772.32 Da        Isoelectric Point: 6.8323

>NTDB_id=486465 IF561_RS15080 WP_100662586.1 3196330..3197346(+) (pilM) [Vibrio parahaemolyticus strain 19-VB00998]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTPQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=486465 IF561_RS15080 WP_100662586.1 3196330..3197346(+) (pilM) [Vibrio parahaemolyticus strain 19-VB00998]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGCCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTAGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.665

99.704

0.485