Detailed information NTG0485189

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: BAINH2_RS05315
• Genome accession: NZ_CP061852
• Coordinates: 1065303..1068803 (+)
• Length: 1166 a.a.
• NCBI ID: WP_053573545.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain INH2-4b
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1060303..1073803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BAINH2_RS05290 (BAINH2_05290)	-	1060486..1061643 (-)	1158	WP_033574498.1	AbrB family transcriptional regulator	-
BAINH2_RS05295 (BAINH2_05295)	-	1061811..1063007 (-)	1197	WP_012117246.1	MFS transporter	-
BAINH2_RS05300 (BAINH2_05300)	-	1063023..1063643 (-)	621	WP_012117247.1	TetR/AcrR family transcriptional regulator	-
BAINH2_RS05305 (BAINH2_05305)	-	1063871..1064380 (-)	510	WP_015417176.1	ferritin-like domain-containing protein	-
BAINH2_RS05310 (BAINH2_05310)	-	1064449..1065163 (-)	715	Protein_996	DUF421 domain-containing protein	-
BAINH2_RS05315 (BAINH2_05315)	addB	1065303..1068803 (+)	3501	WP_053573545.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
BAINH2_RS05320 (BAINH2_05320)	addA	1068790..1072494 (+)	3705	WP_053573573.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
BAINH2_RS05325 (BAINH2_05325)	sbcD	1072559..1073731 (+)	1173	WP_025649562.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 133341.86 Da
• Isoelectric Point: 5.4001

>NTDB_id=485189 BAINH2_RS05315 WP_053573545.1 1065303..1068803(+) (addB) [Bacillus amyloliquefaciens strain INH2-4b]
MGVEFLVGRSGSGKTRLIIDSIQDELRREPFGKPIIFLVPDQMTFLMEYELAKTPDIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTTTGVQMLLRKLIEEHKHEFKVYQKASDKTGFTEQVERMLTEFKRYCIEPEDVRRMAESGTASEYRGERMLSE
KLHDLGILYQQMERSLAGHYLHSEDYLTLLAQQIPLADVVKGARVYVDGFYQFTPQELRVLEQLIMHAEHVTFSLTADSP
SAEQAPDELDLFRMTGSTYYKLYQTAKELNADISCKELHGTKRHQHAPELACIESQYDVRPAAAYTGGQEAFTVMQAQNR
RAELEGIAREIQSLVRDGGYRYKDMAILIRQPEDYKDLLKEVFADYGLPYFIDGKASMQHHPLIEFIRSSLDVVKGNWRY
EAVFRCAKTELLFPLDQPEQKIREQVDQLENYCIAYGIKGERWTSGERFVYRRFVSLDEDFAQTDQEIEMEHMLNETREW
IAAPLVRLQNRMKKAKTVQSMAEALYLFLEDTDVPLKLDRKRQRAEETGNMIDAQQHGQAWDAVIQLLEEFAGMMGEDEI
SLALFQQMLETGTESLHFSLIPPALDQVFVGNMDLSRMYGTSCTFVIGVNDGVLPARPDENGVLSDDDREWLKAVGVELS
SAGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSIVVNRLGELFPDHQEKLSAADPEQVSEEEQLQYLVNKQ
VAQTYTASQLRLWTREYEISDVWWSAYNVLMKESDHRRAKKLFSSLFFRNEAKRLERPVSRQLYGEHIKGSVSRMEAFNA
CQFSHFASHGLQLKERQFFKLDAPDIGQLFHSSLKLISDRLREQKLEWRDLTKDQCRNFSYEAVERLAPKLQKEILLSSN
RHFYVKEKLQKIVTRVSGILSEHAKASGFVPVGLELGFGGSGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDRGLDLAEVYYGLALQMLTYLDLSITHSADWLGMKATPAGVLYFHIHDPMIQASLPMGLDEIEQEIFKKFKMKGL
LLGDREAISLMDTTLEEGRSNIINAGLKKDGSLRSDSAAVSEQDFHLLTDHVRRTFEQAGEAITDGLVSITPYKLKDKTP
CTYCAFKSVCQFDESLKENEYRSLKAEKDGTILDWLKKEADDDANS

Nucleotide

• Length: 3501 bp

>NTDB_id=485189 BAINH2_RS05315 WP_053573545.1 1065303..1068803(+) (addB) [Bacillus amyloliquefaciens strain INH2-4b]
TTGGGTGTTGAGTTTTTAGTAGGCAGATCGGGGAGTGGGAAAACGAGGCTGATTATCGACAGCATTCAAGATGAACTGCG
CCGGGAGCCGTTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCGGATATCGGCGGCATGATACGCGCTCAAGTGTTCAGTTTTTCGCGTTTGGCGTGGCGCGTGCTTCAGCATACCGGG
GGCATGAACCGCCCGTTTGTGACGACGACGGGTGTGCAGATGCTTTTAAGGAAATTGATTGAAGAACATAAGCACGAGTT
TAAAGTGTATCAAAAAGCGAGTGACAAAACCGGGTTCACTGAGCAGGTGGAACGGATGCTGACAGAGTTTAAGCGGTACT
GTATTGAGCCTGAAGACGTCCGCCGCATGGCGGAAAGCGGAACGGCGTCAGAATATCGCGGAGAGCGGATGCTGTCTGAA
AAACTTCATGATTTAGGCATTTTATATCAGCAGATGGAAAGAAGCCTCGCCGGACACTATTTGCATTCAGAGGATTATTT
GACGCTGCTCGCTCAGCAGATTCCTTTGGCGGACGTTGTAAAAGGCGCCCGTGTTTACGTCGACGGATTCTATCAATTTA
CGCCGCAGGAATTAAGGGTGCTGGAGCAGCTGATCATGCATGCTGAACATGTCACATTCTCGCTGACGGCGGACAGCCCT
TCGGCGGAACAGGCACCGGATGAGCTCGATCTGTTCAGAATGACGGGAAGCACATACTATAAGCTTTATCAGACGGCAAA
GGAATTGAATGCCGATATTTCCTGCAAGGAACTGCATGGCACGAAGCGGCATCAGCATGCGCCGGAGCTTGCCTGTATAG
AGTCGCAGTACGATGTGCGTCCCGCCGCGGCGTATACCGGCGGACAGGAAGCTTTTACCGTCATGCAGGCGCAAAACAGA
AGAGCTGAGCTTGAAGGTATTGCCCGGGAAATTCAATCACTTGTCCGGGACGGAGGATACCGGTACAAAGATATGGCGAT
TCTCATCCGTCAGCCGGAAGATTATAAAGATTTGCTGAAGGAAGTGTTTGCCGATTACGGGCTTCCTTATTTTATTGACG
GAAAAGCTTCTATGCAGCATCACCCGCTTATTGAATTCATCCGTTCAAGCCTGGATGTCGTGAAGGGGAACTGGAGATAT
GAAGCGGTGTTCCGCTGTGCGAAAACCGAACTGTTATTCCCGCTTGATCAGCCGGAGCAGAAAATAAGAGAGCAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGCATTAAAGGGGAGCGCTGGACGAGCGGCGAGCGGTTTGTATACAGGCGCT
TCGTATCGCTCGATGAGGATTTCGCGCAGACGGATCAGGAAATAGAGATGGAACACATGCTGAACGAGACGAGAGAGTGG
ATAGCCGCGCCCCTTGTCCGGCTTCAAAACCGGATGAAAAAAGCGAAAACGGTTCAAAGCATGGCGGAGGCTCTTTATCT
CTTTTTGGAAGATACCGATGTTCCCCTGAAGCTGGACCGGAAAAGGCAGCGGGCCGAAGAGACCGGAAACATGATCGATG
CGCAGCAGCACGGCCAGGCGTGGGATGCCGTTATCCAGCTTCTCGAGGAGTTTGCCGGCATGATGGGTGAAGATGAAATA
TCTTTGGCACTGTTCCAGCAAATGCTTGAAACGGGAACGGAATCGCTTCATTTTTCTTTAATTCCGCCTGCGCTTGACCA
AGTATTTGTAGGGAATATGGACCTCTCAAGAATGTACGGCACGTCCTGCACATTCGTCATCGGGGTCAATGACGGCGTTC
TTCCGGCGCGCCCGGATGAAAACGGCGTGCTGTCTGATGATGACCGGGAGTGGCTGAAAGCAGTCGGAGTGGAGCTGTCT
TCCGCGGGGCGTGAGCGGCTGCTTGATGAGCATTTTCTCATCTATATGGCGCTGTCGAGTCCGTCTGACCGTCTTTATGT
GTCATACCCGATTGCTGATGCGGAAGGAAAAACGCTCCTGCCGTCAATCGTTGTAAACCGGCTTGGCGAGCTGTTTCCCG
ATCATCAGGAAAAGCTGTCAGCGGCTGATCCCGAGCAGGTAAGCGAAGAAGAGCAGCTTCAGTATCTCGTCAATAAACAG
GTGGCTCAGACATATACGGCAAGTCAGCTCAGGCTGTGGACAAGAGAGTACGAGATCAGTGACGTATGGTGGAGCGCATA
CAATGTGCTGATGAAAGAGTCTGATCACCGGCGTGCGAAAAAGCTGTTTTCAAGCTTGTTTTTCCGCAATGAGGCGAAGC
GTCTTGAACGGCCTGTTTCGCGCCAGCTTTACGGCGAGCATATTAAAGGAAGCGTCTCCCGGATGGAGGCGTTTAATGCC
TGCCAGTTTTCTCATTTTGCATCTCACGGGCTTCAATTAAAAGAGCGGCAGTTTTTCAAGCTGGACGCGCCGGATATCGG
CCAGCTGTTTCACTCCAGCCTGAAGCTGATTTCAGACCGGCTTCGTGAGCAGAAGCTGGAATGGCGCGATCTGACGAAAG
ATCAATGCCGGAATTTCTCCTATGAAGCGGTGGAACGGCTTGCGCCAAAGCTGCAAAAAGAGATTCTGCTCAGCTCAAAC
AGACACTTTTATGTGAAGGAAAAACTGCAGAAGATCGTCACGCGTGTTTCAGGCATATTAAGCGAGCATGCGAAGGCGAG
CGGTTTCGTCCCGGTCGGTCTTGAACTCGGTTTCGGCGGAAGCGGGCCGCTGCCTCCGCTCACTTTCACGCTGAAAAACG
GCTGTACGATGGAACTCGTCGGACGGATCGACCGCGTGGATAAAGCGGAAAGTTCAAAAGGCCTGCTGCTGAGAATCGTT
GATTATAAATCAAGCGACAGGGGGCTTGATCTGGCTGAGGTGTATTACGGGCTCGCTCTGCAAATGCTCACATATCTTGA
CTTATCGATTACACATTCCGCGGATTGGCTCGGCATGAAAGCGACACCGGCAGGCGTTTTGTATTTCCATATTCACGATC
CGATGATTCAGGCCAGTCTGCCGATGGGGCTTGATGAAATCGAGCAGGAAATTTTCAAGAAATTTAAAATGAAAGGCCTT
CTCCTCGGTGACCGGGAGGCGATCAGTCTGATGGACACGACTTTGGAGGAAGGGCGTTCAAATATCATCAATGCCGGTTT
GAAAAAGGACGGGTCTTTAAGATCCGACTCAGCCGCGGTCAGCGAACAGGACTTTCATCTGCTGACAGACCATGTGCGGC
GGACATTTGAACAGGCTGGCGAAGCGATAACGGACGGGCTTGTGTCCATTACCCCGTATAAGCTGAAAGATAAGACGCCT
TGTACGTACTGTGCGTTTAAGTCGGTATGCCAGTTTGATGAGTCACTTAAGGAAAATGAATACCGCAGCCTGAAAGCGGA
AAAAGACGGCACCATACTGGATTGGCTGAAGAAGGAGGCGGATGATGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (791-1131)

• UvrD-like helicase C-terminal domain (UvrD_C) (303-669)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	83.533	100	0.835