Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   J9247_RS25000 Genome accession   NZ_CP072783
Coordinates   5353198..5353623 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain LICME WGH-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5348198..5358623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9247_RS24985 (J9247_24985) pilX 5348768..5349355 (+) 588 WP_033972921.1 type 4a pilus minor pilin PilX -
  J9247_RS24990 (J9247_24990) pilY1 5349367..5352852 (+) 3486 WP_210224838.1 type 4a pilus biogenesis protein PilY1 -
  J9247_RS24995 (J9247_24995) pilY2 5352854..5353201 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  J9247_RS25000 (J9247_25000) comF 5353198..5353623 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  J9247_RS25005 (J9247_25005) ispH 5353670..5354614 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  J9247_RS25010 (J9247_25010) fkpB 5354700..5355140 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  J9247_RS25015 (J9247_25015) lspA 5355133..5355642 (-) 510 WP_025271359.1 signal peptidase II -
  J9247_RS25020 (J9247_25020) ileS 5355635..5358466 (-) 2832 WP_124120224.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=484936 J9247_RS25000 WP_003094721.1 5353198..5353623(+) (comF) [Pseudomonas aeruginosa strain LICME WGH-6]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=484936 J9247_RS25000 WP_003094721.1 5353198..5353623(+) (comF) [Pseudomonas aeruginosa strain LICME WGH-6]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383