Detailed information NTG0483729

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC778_RS04335
• Genome accession: NZ_CP061634
• Coordinates: 902990..904108 (-)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS41
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 897990..909108

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC778_RS04305 (IC778_04300)	-	898820..899668 (+)	849	WP_077170048.1	class II glutamine amidotransferase	-
IC778_RS04310 (IC778_04305)	-	899919..900374 (+)	456	WP_004703295.1	bacteriohemerythrin	-
IC778_RS04315 (IC778_04310)	-	900527..901594 (+)	1068	WP_143221137.1	hypothetical protein	-
IC778_RS04320 (IC778_04315)	-	901594..901917 (+)	324	WP_019456825.1	RnfH family protein	-
IC778_RS04325 (IC778_04320)	-	901973..902371 (-)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC778_RS04330 (IC778_04325)	fur	902483..902920 (+)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC778_RS04335 (IC778_04330)	pilU	902990..904108 (-)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC778_RS04340 (IC778_04335)	pilT	904136..905173 (-)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC778_RS04345 (IC778_04340)	-	905300..905992 (+)	693	WP_077170049.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC778_RS04350 (IC778_04345)	-	906099..906941 (+)	843	WP_019456821.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483729 IC778_RS04335 WP_004703285.1 902990..904108(-) (pilU) [Acinetobacter seifertii strain AS41]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483729 IC778_RS04335 WP_004703285.1 902990..904108(-) (pilU) [Acinetobacter seifertii strain AS41]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401