Detailed information NTG0483483

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IC787_RS04430
• Genome accession: NZ_CP061598
• Coordinates: 920863..921981 (-)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain AS58
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 915863..926981

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC787_RS04400 (IC787_04395)	-	916694..917542 (+)	849	WP_077170048.1	class II glutamine amidotransferase	-
IC787_RS04405 (IC787_04400)	-	917793..918247 (+)	455	Protein_846	bacteriohemerythrin	-
IC787_RS04410 (IC787_04405)	-	918400..919467 (+)	1068	WP_143221137.1	hypothetical protein	-
IC787_RS04415 (IC787_04410)	-	919467..919790 (+)	324	WP_019456825.1	RnfH family protein	-
IC787_RS04420 (IC787_04415)	-	919846..920244 (-)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
IC787_RS04425 (IC787_04420)	fur	920356..920793 (+)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
IC787_RS04430 (IC787_04425)	pilU	920863..921981 (-)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IC787_RS04435 (IC787_04430)	pilT	922009..923046 (-)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
IC787_RS04440 (IC787_04435)	-	923173..923865 (+)	693	WP_077170049.1	YggS family pyridoxal phosphate-dependent enzyme	-
IC787_RS04445 (IC787_04440)	-	923972..924815 (+)	844	Protein_854	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=483483 IC787_RS04430 WP_004703285.1 920863..921981(-) (pilU) [Acinetobacter seifertii strain AS58]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=483483 IC787_RS04430 WP_004703285.1 920863..921981(-) (pilU) [Acinetobacter seifertii strain AS58]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTCGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAGCGTGATATGCCGGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCTTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATTATTACTATTGAA
GACCCTATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTAGGTATTGATACTGACTCGTTCGA
AATTGCTTTAAAAAATACGTTACGACAAGCGCCCGATGTCATCTTAATTGGTGAGATCCGCTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTAACTTTTGATCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401