Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ICJ61_RS06320 Genome accession   NZ_CP061284
Coordinates   1222893..1226393 (+) Length   1166 a.a.
NCBI ID   WP_188323669.1    Uniprot ID   -
Organism   Bacillus halotolerans strain XH-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1217893..1231393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICJ61_RS06300 (ICJ61_06305) - 1218746..1219948 (-) 1203 WP_188323668.1 MFS transporter -
  ICJ61_RS06305 (ICJ61_06310) - 1220064..1221794 (+) 1731 WP_106022374.1 ABC transporter substrate-binding protein -
  ICJ61_RS06310 (ICJ61_06315) - 1221828..1222142 (-) 315 WP_044156484.1 four-helix bundle copper-binding protein -
  ICJ61_RS06315 (ICJ61_06320) - 1222271..1222708 (-) 438 WP_044156482.1 ferritin family protein -
  ICJ61_RS06320 (ICJ61_06325) addB 1222893..1226393 (+) 3501 WP_188323669.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ICJ61_RS06325 (ICJ61_06330) addA 1226380..1230084 (+) 3705 WP_188323670.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ICJ61_RS06330 (ICJ61_06335) sbcD 1230156..1231334 (+) 1179 WP_106020584.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134800.76 Da        Isoelectric Point: 5.3837

>NTDB_id=481504 ICJ61_RS06320 WP_188323669.1 1222893..1226393(+) (addB) [Bacillus halotolerans strain XH-1]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERVLSE
KLHDLSILYQQMEKSLEDQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMFQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQTAKELNLDITYKELDESKRHKRAPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKDYRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKTSMLNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNQPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEIENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGVDDI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKIDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRLLKAEKDKTVLDWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=481504 ICJ61_RS06320 WP_188323669.1 1222893..1226393(+) (addB) [Bacillus halotolerans strain XH-1]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGACGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGGGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAGGATCAGTATCTTCATTCTGAGGATTATTT
GACGCTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGGGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGTTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCCTCCACCAGACGGCAAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTTGATGAATCAAAGCGGCACAAGCGCGCACCGGAATTAGCCCATTTAG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACCGTGATGCAGGCCGCAAACAGA
CGAGCAGAGCTGGAAGGTATTGCCCGGGAAATCCACAGCCTTGTCAGAGAGAAGGACTACCGTTACAAGGATATGGCGAT
TCTAGCGCGTCAGCCGGAAGACTATAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACATCTATGCTGAACCACCCGTTAGTAGAATTTATCCGGTCAAGCCTTGACGTATTAAAGGGAAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTTCCGCTTAACCAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGGATCAAAGGCGATCGCTGGACAAAGGGTGATCGTTTCCATTACAGACGTT
TTGTGTCGTTAGATGATGAATTTGCGCAGACCGATCAAGAAATCGAAATAGAAAACATGCTGAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGCGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CGCAGCAGCACCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGTAGATGACATT
TCTCTCGATTTGTTTCAGCAAATGATGGAGACTGGTGCAGAGTCGCTGACATTCTCTCTCATCCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATATACGGCACCTCATGCACCTTTGTGATCGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGACGAAAACGGGGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATAGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGCTTTCAAGCCCGTCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGGAAAACGCTTTTGCCGTCGATTGTAATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGTGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGCGATGAGGAACAGCTTATGTACCTTGTCAATAAAAGC
GTGGCGCAATCATACACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAGATCAGCGACGTCTGGTGGAGCGCGTA
TAATGCGCTGATGAATGAACCTGACAGGCTGGAATCGAAAAAGCTTTTCTCTAGCCTGTTTTTCCGAAATGAAGTCAAGC
AGCTTGAACGCTCTGTATCGAGACAGCTCTATGGTGAACGCATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGCCCGTTCTCCCATTTTGCGTCCCACGGGCTGCATCTGAAGGAACGTCAATTTTTCAAGCTTGAAGCACCGGATATCGG
GCAGCTGTTTCACTCCAGCTTAAAACTGATTTCAGACCGGCTTCGTGAGCAAAAAATAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTCGAGCGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTATCCGGCATTTTAAGCGAGCATGCAAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGAATTGTC
GACTATAAATCCAGCGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TTTATCGATTACACATTCGTTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATCCAATCGAATCTTCCGCTCGGACTTGATGAAATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTTGGTGATCAGGAAGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCCT
GAAGAAAGACGGCTCACTCAGATCGGATTCAGCGGCAGTCGGTGAAAAAGAATTTGAACTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCT
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCTGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-555)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

94.34

100

0.943