NTDB

Overview

Name	pilC	Type	Machinery gene
Locus tag	ICJ04_RS04555	Genome accession	NZ_CP061204
Coordinates	1089039..1090298 (-)	Length	419 a.a.
NCBI ID	WP_188326364.1	Uniprot ID	A0A7H1AP65
Organism	Stenotrophomonas sp. 169
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1078761..1103181	1089039..1090298	within	0

Gene organization within MGE regions

Location: 1078761..1103181

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ICJ04_RS04510 (ICJ04_04510)	-	1078761..1082015 (-)	3255	WP_188326355.1	transporter substrate-binding domain-containing protein	-
ICJ04_RS04515 (ICJ04_04515)	rimK	1082309..1083187 (+)	879	WP_188326356.1	30S ribosomal protein S6--L-glutamate ligase	-
ICJ04_RS04520 (ICJ04_04520)	-	1083537..1084031 (+)	495	WP_188326357.1	hypothetical protein	-
ICJ04_RS04525 (ICJ04_04525)	-	1084178..1084855 (+)	678	WP_188326358.1	response regulator transcription factor	-
ICJ04_RS04530 (ICJ04_04530)	-	1084848..1086191 (+)	1344	WP_188326359.1	HAMP domain-containing sensor histidine kinase	-
ICJ04_RS04535 (ICJ04_04535)	-	1086301..1086714 (-)	414	WP_188326360.1	hypothetical protein	-
ICJ04_RS04540 (ICJ04_04540)	-	1087158..1087508 (+)	351	WP_188326361.1	transcriptional regulator	-
ICJ04_RS04545 (ICJ04_04545)	coaE	1087558..1088175 (-)	618	WP_188326362.1	dephospho-CoA kinase	-
ICJ04_RS04550 (ICJ04_04550)	-	1088172..1089035 (-)	864	WP_188326363.1	A24 family peptidase	-
ICJ04_RS04555 (ICJ04_04555)	pilC	1089039..1090298 (-)	1260	WP_188326364.1	type II secretion system F family protein	Machinery gene
ICJ04_RS04560 (ICJ04_04560)	pilA	1090654..1091058 (+)	405	WP_188326365.1	pilin	Machinery gene
ICJ04_RS04565 (ICJ04_04565)	-	1091336..1094383 (+)	3048	WP_188326366.1	fused MFS/spermidine synthase	-
ICJ04_RS04570 (ICJ04_04570)	pilB	1095069..1096793 (+)	1725	WP_188326367.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ICJ04_RS04575 (ICJ04_04575)	-	1096960..1097667 (+)	708	WP_188326368.1	YitT family protein	-
ICJ04_RS04580 (ICJ04_04580)	-	1097797..1098333 (-)	537	WP_188326369.1	DNA-3-methyladenine glycosylase I	-
ICJ04_RS04585 (ICJ04_04585)	-	1098330..1100126 (-)	1797	WP_188326370.1	DUF4153 domain-containing protein	-
ICJ04_RS04590 (ICJ04_04590)	-	1100208..1100774 (+)	567	WP_188326371.1	YqgE/AlgH family protein	-
ICJ04_RS04595 (ICJ04_04595)	ruvX	1100767..1101234 (+)	468	WP_188326372.1	Holliday junction resolvase RuvX	-
ICJ04_RS04600 (ICJ04_04600)	-	1101251..1102201 (+)	951	WP_188326373.1	aspartate carbamoyltransferase catalytic subunit	-
ICJ04_RS04605 (ICJ04_04605)	-	1102237..1103181 (-)	945	WP_188326374.1	hypothetical protein	-

Sequence

Protein

Download Length: 419 a.a. Molecular weight: 45749.20 Da Isoelectric Point: 10.2269

                                >NTDB_id=481329 ICJ04_RS04555 WP_188326364.1 1089039..1090298(-) (pilC) [Stenotrophomonas sp. 169]

MSASRSAMKKEPVARSTTEMAPFVWEGTDKRGVKMKGEQLAKNANLLKAELRRQGITPKIVKPKSKPLFGAAGKPVSAKD

IAFFSRQMATMMKSGVPIVSSLEIIGGGHKNPRMKKMVDSIRTDIEGGSSLHEAISRHPVQFDELYRNLVRAGEGAGVLE

TVLDTVANYKENIEALKGKIKKALFYPAMVMAVALIVSSILLLFVVPQFEEVFAGFGAELPAFTQIIVNMSRFMTSWWWL

IAIIIAGVIVGSIMAYKRSPKMQHTMDRFILKVPVIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYEE

GVLRMRDDVSVGYPVNMAMKQINLFPHMVVQMTAIGEEAGALDTMLFKVAEYFEQEVNNAVDSLSSLLEPMIMVFIGVVV

GGMVIGMYLPIFKLGAVVG

Nucleotide

Download Length: 1260 bp

                                >NTDB_id=481329 ICJ04_RS04555 WP_188326364.1 1089039..1090298(-) (pilC) [Stenotrophomonas sp. 169]

ATGTCCGCAAGTCGCAGTGCGATGAAGAAAGAGCCCGTGGCGCGCAGCACCACGGAGATGGCACCGTTTGTCTGGGAGGG

GACCGACAAGCGGGGCGTGAAGATGAAAGGTGAGCAGTTGGCCAAGAATGCCAATCTGCTCAAGGCAGAGCTTCGACGTC

AGGGGATCACACCCAAGATCGTGAAACCAAAGAGCAAGCCGCTGTTTGGTGCGGCAGGCAAGCCTGTGTCTGCTAAAGAT

ATCGCCTTCTTCAGTCGTCAGATGGCGACTATGATGAAATCCGGCGTACCAATTGTCTCGTCATTGGAGATCATTGGCGG

TGGTCATAAGAACCCCCGGATGAAGAAGATGGTGGACTCAATTCGAACGGATATTGAAGGGGGTTCGTCGCTACATGAGG

CCATCAGCAGGCATCCGGTACAGTTCGACGAGCTGTATCGAAACCTGGTCCGAGCTGGTGAAGGGGCTGGTGTGCTGGAG

ACGGTGCTCGATACGGTCGCCAATTACAAAGAGAACATTGAGGCGTTGAAGGGCAAGATCAAGAAGGCATTGTTCTACCC

TGCGATGGTCATGGCAGTTGCTCTTATCGTTTCGTCAATCCTTCTGCTTTTCGTGGTTCCCCAGTTCGAAGAAGTGTTCG

CCGGCTTCGGGGCCGAGCTTCCCGCGTTTACTCAGATCATTGTCAATATGTCGCGTTTCATGACGTCGTGGTGGTGGCTG

ATCGCGATCATAATCGCGGGAGTGATCGTCGGCTCCATCATGGCCTACAAGCGCTCTCCTAAAATGCAGCACACCATGGA

CCGGTTCATCCTCAAGGTCCCGGTCATCGGCCAGATCATGCACAACAGCTCGATCGCCCGCTTCGCCCGCACCACGGCGG

TAACCTTCAAAGCCGGTGTCCCGCTGGTCGAAGCCCTCGGCATCGTCGCAGGCGCTACCGGCAACAAGGTGTACGAAGAA

GGCGTGCTGCGTATGCGTGACGACGTGTCGGTCGGCTACCCGGTCAACATGGCCATGAAGCAGATCAACCTGTTCCCGCA

CATGGTGGTGCAGATGACCGCCATCGGCGAAGAGGCCGGCGCCCTGGACACCATGCTGTTCAAGGTGGCGGAGTACTTCG

AGCAAGAGGTCAACAACGCCGTCGATTCGCTCAGCAGCCTGCTGGAACCGATGATCATGGTCTTCATTGGCGTGGTCGTT

GGCGGTATGGTGATCGGCATGTACTTGCCCATCTTCAAACTCGGCGCGGTGGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(288-410)

Type II secretion system (T2SS), protein F (T2SSF)

(84-207)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A7H1AP65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilC	Pseudomonas stutzeri DSM 10701	51.97	96.897	0.504
pilC	Legionella pneumophila strain ERS1305867	50.248	96.42	0.484
pilC	Acinetobacter baumannii D1279779	50.75	95.465	0.484
pilC	Acinetobacter baylyi ADP1	49.507	96.897	0.48
pilG	Neisseria gonorrhoeae MS11	43.467	94.988	0.413
pilG	Neisseria meningitidis 44/76-A	42.857	95.227	0.408
pilC	Vibrio cholerae strain A1552	40.887	96.897	0.396
pilC	Vibrio campbellii strain DS40M4	40.547	95.943	0.389

Detailed information NTG0481329